ArticleGenes & development2002
An mt(+) gamete-specific nuclease that targets mt(-) chloroplasts during sexual reproduction in C. reinhardtii.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 81 citations in OpenAlex.
- Global identification of AGO3-RNA interactions reveals targets of small RNA-mediated gene regulation in Chlamydomonas reinhardtii.Plant & cell physiology · 2025Article
- Cytoplasmic inheritance: The transmission of plastid and mitochondrial genomes across cells and generations.Plant physiology · 2025Review
- Modes and mechanisms for the inheritance of mitochondria and plastids in pathogenic protists.PLoS pathogens · 2025Review
- Sex-linked deubiquitinase establishes uniparental transmission of chloroplast DNA.Nature communications · 2022Article
- HBD1 protein with a tandem repeat of two HMG-box domains is a DNA clip to organize chloroplast nucleoids inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- A functional bacteria-derived restriction modification system in the mitochondrion of a heterotrophic protist.PLoS biology · 2021Article
- Cytoplasmic inheritance of mitochondria and chloroplasts in the anisogamous brown alga Mutimo cylindricus (Phaeophyceae).Protoplasma · 2021Article
- Step-wise elimination of α-mitochondrial nucleoids and mitochondrial structure as a basis for the strict uniparental inheritance in Cryptococcus neoformans.Scientific reports · 2020Article
- The CONSTANS flowering complex controls the protective response of photosynthesis in the green alga Chlamydomonas.Nature communications · 2019Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Dynamic Interplay between Nucleoid Segregation and Genome Integrity in Chlamydomonas Chloroplasts.Plant physiology · 2016Article
- Surfing the vegetal pole in a small population: extracellular vertical transmission of an 'intracellular' deep-sea clam symbiont.Royal Society open science · 2016Article
- Dynamic Changes in the Transcriptome and Methylome of Chlamydomonas reinhardtii throughout Its Life Cycle.Plant physiology · 2015Article
- Eukaryotic Components Remodeled Chloroplast Nucleoid Organization during the Green Plant Evolution.Genome biology and evolution · 2015Article
- Origins of eukaryotic sexual reproduction.Cold Spring Harbor perspectives in biology · 2014Review
- Chloroplast DNA replication is regulated by the redox state independently of chloroplast division in Chlamydomonas reinhardtii.Plant physiology · 2013Article
- A conserved, Mg²+-dependent exonuclease degrades organelle DNA during Arabidopsis pollen development.The Plant cell · 2011Article
- Review of cytological studies on cellular and molecular mechanisms of uniparental (maternal or paternal) inheritance of plastid and mitochondrial genomes induced by active digestion of organelle nuclei (nucleoids).Journal of plant research · 2010Review
- Uniparental inheritance of cpDNA and the genetic control of sexual differentiation in Chlamydomonas reinhardtii.Journal of plant research · 2010Review
- Cytoplasmic inheritance in green algae: patterns, mechanisms and relation to sex type.Journal of plant research · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the active digestion of mating-type minus (mt-) chloroplast DNA (cpDNA) in young zygotes is considered to be the basis for the uniparental inheritance of cpDNA in Chlamydomonas reinhardtii, little is known about the underlying molecular mechanism. One model of active digestion proposes that nucleases are either synthesized or activated to digest mt- cpDNA. We used a native-PAGE/in gelo assay to investigate nuclease activities in chloroplasts from young zygotes, and identified a novel Ca(2+)-dependent nuclease activity. The timing of activation (approximately 60-90 min after mating) and the localization of the nuclease activity (in mt- chloroplasts) coincided with the active digestion of mt- cpDNA. Furthermore, the activity of the nuclease was coregulated with the maturation of mating-type plus (mt+) gametes, which would enable the efficient digestion of mt- cpDNA. Based on these observations, we propose that the nuclease (designated as Mt(+)-specific DNase, MDN) is a developmentally controlled nuclease that is activated in mt+ gametes and participates in the destruction of mt- cpDNA in young zygotes, thereby ensuring uniparental inheritance of chloroplast traits.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.