ArticleThe American journal of pathology2002
Diminished callus size and cartilage synthesis in alpha 1 beta 1 integrin-deficient mice during bone fracture healing.
Article in The American journal of pathology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 87 citations in OpenAlex.
- Optimizing Tissue-Engineered Periosteum Biochemical Cues to Hasten Bone Allograft Healing.Journal of biomedical materials research. Part A · 2025Article
- Discoidin domain receptor 2 is an important modulator of BMP signaling during heterotopic bone formation.Bone research · 2025Article
- Effects of self-assembled type II collagen fibrils on the morphology and growth of pre-chondrogenic ATDC5 cells.Osteoarthritis and cartilage open · 2024Article
- Depleting transforming growth factor beta receptor 2 signalling in the cartilage ofOsteoarthritis and cartilage open · 2023Article
- Transcriptomic analysis of the cerebral hippocampal tissue in spontaneously hypertensive rats exposed to acute hypobaric hypoxia: associations with inflammation and energy metabolism.Scientific reports · 2023Article
- Roles of focal adhesion proteins in skeleton and diseases.Acta pharmaceutica Sinica. B · 2023Review
- Targeting integrin pathways: mechanisms and advances in therapy.Signal transduction and targeted therapy · 2023Review
- Discoidin domain receptors; an ancient family of collagen receptors has major roles in bone development, regeneration and metabolism.Frontiers in dental medicine · 2023Article
- Targeted activation of androgen receptor signaling in the periosteum improves bone fracture repair.Cell death & disease · 2022Article
- Role of Discoidin Domain Receptor 2 in Craniofacial Bone Regeneration.Journal of dental research · 2021Article
- Review of Integrin-Targeting Biomaterials in Tissue Engineering.Advanced healthcare materials · 2020Review
- A rare cardiac phenotype of dextrocardia observed in a fetus with 1p36 deletion syndrome and a balanced translocation: a prenatal case report.Molecular cytogenetics · 2020Article
- BDNF promoted osteoblast migration and fracture healing by up-regulating integrin β1 via TrkB-mediated ERK1/2 and AKT signalling.Journal of cellular and molecular medicine · 2020Article
- Reciprocal Interplay Between Fibrillar Collagens and Collagen-Binding Integrins: Implications in Cancer Progression and Metastasis.Frontiers in oncology · 2020Review
- Role of prolyl hydroxylation in the molecular interactions of collagens.Essays in biochemistry · 2019Review
- A comprehensive characterisation of large-scale expanded human bone marrow and umbilical cord mesenchymal stem cells.Stem cell research & therapy · 2019Article
- Proline hydroxylation in collagen supports integrin binding by two distinct mechanisms.The Journal of biological chemistry · 2018Article
- Control of the Osteoblast Lineage by Mitogen-Activated Protein Kinase Signaling.Current molecular biology reports · 2017Article
- Sika Deer Antler Collagen Type I-Accelerated Osteogenesis in Bone Marrow Mesenchymal Stem Cells via the Smad Pathway.Evidence-based complementary and alternative medicine : eCAM · 2016Article
- Diabetes, diabetic complications, and fracture risk.Current osteoporosis reports · 2015Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Integrins mediate cell adhesion to extracellular matrix components. Integrin alpha 1 beta 1 is a collagen receptor expressed on many mesenchymal cells, but mice deficient in alpha 1 integrin (alpha1-KO) have no gross structural defects. Here, the regeneration of a fractured long bone was studied in alpha1-KO mice. These mice developed significantly less callus tissue than the wild-type (WT) mice, and safranin staining revealed a defect in cartilage formation. The mRNA levels of nine extracellular matrix genes in calluses were evaluated by Northern blotting. During the first 9 days the mRNA levels of cartilage-related genes, including type II collagen, type IX collagen, and type X collagen, were lower in alpha1-KO mice than in WT mice, consistent with the reduced synthesis of cartilaginous matrix appreciated in tissue sections. Histological observations also suggested a diminished number of chondrocytes in the alpha 1-KO callus. Proliferating cell nuclear antigen staining revealed a reduction of mesenchymal progenitors at the callus site. Although, the number of mesenchymal stem cells (MSCs) obtained from WT and alpha 1-KO whole marrow was equal, in cell culture the proliferation rate of the MSCs of alpha 1-KO mice was slower, recapitulating the in vivo observation of reduced callus cell proliferation. The results demonstrate the importance of proper collagen-integrin interaction in fracture healing and suggest that alpha1 integrin plays an essential role in the regulation of MSC proliferation and cartilage production.
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