ArticlePlant molecular biology2002
Identification of a tRNA isopentenyltransferase gene from Arabidopsis thaliana.
Article in Plant molecular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 68 citations in OpenAlex.
- Spatially distributed cytokinins: Metabolism, signaling, and transport.Plant communications · 2024Review
- Identification of IPT9 in Brachiaria brizantha (syn. Urochloa brizantha) and expression analyses during ovule development in sexual and apomictic plants.Molecular biology reports · 2023Article
- Identification and Expression Analysis of the Isopentenyl Transferase (IPT) Gene Family under Lack of Nitrogen Stress in Oilseed (Plants (Basel, Switzerland) · 2023Article
- The Importance of Cytokinins during Reproductive Development in Arabidopsis and Beyond.International journal of molecular sciences · 2020Review
- Targeting mitochondrial and cytosolic substrates of TRIT1 isopentenyltransferase: Specificity determinants and tRNA-i6A37 profiles.PLoS genetics · 2020Article
- Flower-Specific Overproduction of Cytokinins Altered Flower Development and Sex Expression in the Perennial Woody PlantInternational journal of molecular sciences · 2020Article
- Phytohormone metabolism in human cells: Cytokinins are taken up and interconverted in HeLa cell culture.FASEB bioAdvances · 2019Article
- Manipulation of cytokinin level in the ergot fungus Claviceps purpurea emphasizes its contribution to virulence.Current genetics · 2018Article
- An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity.Journal of visualized experiments : JoVE · 2018Article
- Article
- Deciphering the epitranscriptome: A green perspective.Journal of integrative plant biology · 2016Review
- Adaptional evolution of trichome inEcology and evolution · 2016Article
- Cytokinin Production by the Rice Blast Fungus Is a Pivotal Requirement for Full Virulence.PLoS pathogens · 2016Article
- Review
- Isopentenyltransferase-1 (IPT1) knockout in Physcomitrella together with phylogenetic analyses of IPTs provide insights into evolution of plant cytokinin biosynthesis.Journal of experimental botany · 2014Article
- Mod5 protein binds to tRNA gene complexes and affects local transcriptional silencing.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Identification of two additional members of the tRNA isopentenyltransferase family in Physcomitrella patens.Plant molecular biology · 2013Article
- Article
- Roles of Trm9- and ALKBH8-like proteins in the formation of modified wobble uridines in Arabidopsis tRNA.Nucleic acids research · 2011Article
- Characterization of cytokinin and adenine transport in Arabidopsis cell cultures.Plant physiology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tRNA of most organisms contain modified adenines called cytokinins. Situated next to the anticodon, they have been shown to influence translational fidelity and efficiency. The enzyme that synthesizes cytokinins on pre-tRNA, tRNA isopentenyltransferase (EC 2.5.1.8), has been studied in micro-organisms like Escherichia coli and SaccharomYces cerevisiae, and the corresponding genes have been cloned. We here report the first cloning and functional characterization of a homologous gene from a plant, Arabidopsis thaliana. Expression in S. cerevisiae showed that the gene can complement the anti-suppressor phenotype of a mutant that lacks MOD5, the intrinsic tRNA isopentenyltransferase gene. This was accompanied by the reintroduction of isopentenyladenosine in the tRNA. The Arabidopsis gene is constitutively expressed in seedling tissues.
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