ArticleMolecular and cellular biology2002
Strand bias in targeted gene repair is influenced by transcriptional activity.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 82 citations in OpenAlex.
- The RNA structurome in the asexual blood stages of malaria pathogenRNA biology · 2021Article
- Editing the Genome Without Double-Stranded DNA Breaks.ACS chemical biology · 2018Review
- Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides.Scientific reports · 2016Article
- λ Recombination and Recombineering.EcoSal Plus · 2016Review
- Genetic spell-checking: gene editing using single-stranded DNA oligonucleotides.Plant biotechnology journal · 2016Review
- Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems.PloS one · 2015Article
- Combinatorial gene editing in mammalian cells using ssODNs and TALENs.Scientific reports · 2014Article
- Emerging gene editing strategies for Duchenne muscular dystrophy targeting stem cells.Frontiers in physiology · 2014Review
- Article
- Yeast oligo-mediated genome engineering (YOGE).ACS synthetic biology · 2013Article
- Strand bias influences the mechanism of gene editing directed by single-stranded DNA oligonucleotides.Nucleic acids research · 2011Article
- Oligonucleotides stimulate genomic alterations of Legionella pneumophila.Molecular microbiology · 2011Article
- Progress and prospects: oligonucleotide-directed gene modification in mouse embryonic stem cells: a route to therapeutic application.Gene therapy · 2011Review
- An update on targeted gene repair in mammalian cells: methods and mechanisms.Journal of biomedical science · 2011Review
- PCRless library mutagenesis via oligonucleotide recombination in yeast.Protein science : a publication of the Protein Society · 2010Article
- Parameters of oligonucleotide-mediated gene modification in mouse ES cells.Journal of cellular and molecular medicine · 2010Article
- AAV recombineering with single strand oligonucleotides.PloS one · 2009Article
- DNA breakage associated with targeted gene alteration directed by DNA oligonucleotides.Mutation research · 2009Article
- Genetic correction of splice site mutation in purified and enriched myoblasts isolated from mdx5cv mice.BMC molecular biology · 2009Article
- Single-stranded oligonucleotide-mediated in vivo gene repair in the rd1 retina.Molecular vision · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Modified single-stranded DNA oligonucleotides can direct nucleotide exchange in Saccharomyces cerevisiae. Point and frameshift mutations are corrected in a reaction catalyzed by cellular enzymes involved in various DNA repair processes. The present model centers on the annealing of the vector to one strand of the helix, followed by the correction of the designated base. The choice of which strand to target is a reaction parameter that can be controlled, so here we investigate the properties of strand bias in targeted gene repair. An in vivo system has been established in which a plasmid containing an actively transcribed, but mutated, hygromycin-enhanced green fluorescent protein fusion gene is targeted for repair and upon conversion will confer hygromycin resistance on the cell. Overall transcriptional activity has a positive influence on the reaction, elevating the frequency. If the targeting vector is synthesized so that it directs nucleotide repair on the nontranscribed strand, the level of gene repair is higher than if the template strand is targeted. We provide data showing that the targeting vector can be displaced from the template strand by an active T7 phage RNA polymerase. The strand bias is not influenced by which strand serves as the leading or lagging strand during DNA synthesis. These results may provide an explanation for the enhancement of gene repair observed when the non-template strand is targeted.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.