ArticleMolecular and cellular biology2002
tRNAs in Trypanosoma brucei: genomic organization, expression, and mitochondrial import.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Towards CRISPR-based editing of the mitochondrial genome in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Genomic Organization of Trypanosoma cruzi tRNA Genes.Genome biology and evolution · 2025Article
- Massive RNA Editing in Ascetosporean Mitochondria.Microbes and environments · 2025Article
- Photosynthetic demands on translational machinery drive retention of redundant tRNA metabolism in plant organelles.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Current Status of Regulatory Non-Coding RNAs Research in the Tritryp.Non-coding RNA · 2022Review
- Nuclear tRNA export in trypanosomes: a journey full of twists and turns guided by tRNA modifications.Parasitology · 2021Review
- tRNA import across the mitochondrial inner membrane in T. brucei requires TIM subunits but is independent of protein import.Nucleic acids research · 2020Article
- Article
- The single CCA-adding enzyme ofThe Journal of biological chemistry · 2020Article
- Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic BicosoecidMicroorganisms · 2019Article
- Inducible orthogonal aminoacylation demonstrates that charging is required for mitochondrial tRNA import in Trypanosoma brucei.Scientific reports · 2019Article
- The Complete Mitochondrial Genome ofGenes · 2019Article
- Article
- tRNAs and proteins use the same import channel for translocation across the mitochondrial outer membrane of trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mitochondrial genome diversity in dagger and needle nematodes (Nematoda: Longidoridae).Scientific reports · 2017Article
- An Atypical Mitochondrial Carrier That Mediates Drug Action in Trypanosoma brucei.PLoS pathogens · 2015Article
- Mitochondrial protein import receptors in Kinetoplastids reveal convergent evolution over large phylogenetic distances.Nature communications · 2015Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- A map of 5-methylcytosine residues in Trypanosoma brucei tRNA revealed by sodium bisulfite sequencing.Molecular and biochemical parasitology · 2014Article
- A multiple aminoacyl-tRNA synthetase complex that enhances tRNA-aminoacylation in African trypanosomes.Molecular and cellular biology · 2013Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitochondrial genome of Trypanosoma brucei does not encode tRNAs. Consequently, all mitochondrial tRNAs are imported from the cytosol and originate from nucleus-encoded genes. Analysis of all currently available T. brucei sequences revealed that its genome carries 50 tRNA genes representing 40 different isoacceptors. The identified set is expected to be nearly complete since all but four codons are accounted for. The number of tRNA genes in T. brucei is very low for a eukaryote and lower than those of many prokaryotes. Using quantitative Northern analysis we have determined the absolute abundance in the cell and the mitochondrion of a group of 15 tRNAs specific for 12 amino acids. Except for the initiator type tRNA(Met), which is cytosol specific, the cytosolic and the mitochondrial sets of tRNAs were qualitatively identical. However, the extent of mitochondrial localization was variable for the different tRNAs, ranging from 1 to 7.5% per cell. Finally, by using transgenic cell lines in combination with quantitative Northern analysis it was shown that import of tRNA(Leu)(CAA) is independent of its 5'-genomic context, suggesting that the in vivo import substrate corresponds to the mature, fully processed tRNA.
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