ArticleProceedings of the National Academy of Sciences of the United States of America2002
Microrheometry of semiflexible actin networks through enforced single-filament reptation: frictional coupling and heterogeneities in entangled networks.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 53 citations in OpenAlex.
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Dynamic cross-correlations between entangled biofilaments as they diffuse.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Reversibility and Viscoelastic Properties of Micropillar Supported and Oriented Magnesium Bundled F-Actin.PloS one · 2015Article
- Power-law creep behavior of a semiflexible chain.Physical review. E, Statistical, nonlinear, and soft matter physics · 2008Article
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2 authors at 1 institution in 1 country.
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Abstract
Magnetic tweezers are applied to study the enforced motion of single actin filaments in entangled actin networks to gain insight into friction-mediated entanglement in semiflexible macromolecular networks. Magnetic beads are coupled to one chain end of test filaments, which are pulled by 5 to 20 pN force pulses through entangled solutions of nonlabeled actin, the test filaments thus acting as linear force probes of the network. The transient filament motion is analyzed by microfluorescence, and the deflection-versus-time curves of the beads are evaluated in terms of a mechanical equivalent circuit to determine viscoelastic parameters, which are then interpreted in terms of viscoelastic moduli of the network. We demonstrate that the frictional coefficient characterizing the hydrodynamic coupling of the filaments to the surrounding network is much higher than predicted by the tube model, suggesting that friction-mediated interfilament coupling plays an important role in the entanglement of non-cross-linked actin networks. Furthermore, the local tube width along the filament contour (measured in terms of the root-mean-square displacement characterizing the lateral Brownian motion of the test filament) reveals strong fluctuations that can lead to transient local pinching of filaments.
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