ArticleJournal of virology2002
Folding and dimerization of tick-borne encephalitis virus envelope proteins prM and E in the endoplasmic reticulum.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
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Who cites it
108 citing papers in PubMed, 255 citations in OpenAlex.
- Application of Orthoflavivirus Pseudovirus Technology in Antiviral Research.International journal of molecular sciences · 2026Review
- An Updated Review of Potential Drug Targets for Japanese Encephalitis.Central nervous system agents in medicinal chemistry · 2026Review
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- Variation in virion phosphatidylserine content drives differential GAS6 binding among closely related flaviviruses.Journal of virology · 2025Article
- PARP-16 regulates the PERK and IRE-1α Mediated Unfolded Protein Response in Japanese Encephalitis Virus-Infected Neural Stem/Progenitor Cells.Molecular neurobiology · 2025Article
- Animal Models, Therapeutics, and Vaccine Approaches to Emerging and Re-Emerging Flaviviruses.Viruses · 2024Review
- pr-independent biogenesis of infectious mature Zika virus particles.bioRxiv : the preprint server for biology · 2024Article
- Recent advances in the study of zika virus structure, drug targets, and inhibitors.Frontiers in pharmacology · 2024Review
- N-linked glycosylation of flavivirus E protein contributes to viral particle formation.PLoS pathogens · 2023Article
- Tick-Borne Encephalitis Virus: A Comprehensive Review of Transmission, Pathogenesis, Epidemiology, Clinical Manifestations, Diagnosis, and Prevention.Microorganisms · 2023Review
- Review
- Impact of structural dynamics on biological functions of flaviviruses.The FEBS journal · 2023Review
- Flavivirus prM interacts with MDA5 and MAVS to inhibit RLR antiviral signaling.Cell & bioscience · 2023Article
- Generation and characterization of chimeric Tembusu viruses containing pre-membrane and envelope genes of Japanese encephalitis virus.Frontiers in microbiology · 2023Article
- Identification and characterization of key residues in Zika virus envelope protein for virus assembly and entry.Emerging microbes & infections · 2022Article
- Evolution and activation mechanism of the flavivirus class II membrane-fusion machinery.Nature communications · 2022Article
- Impact of yellow fever virus envelope protein on wild-type and vaccine epitopes and tissue tropism.NPJ vaccines · 2022Article
- Replication is the key barrier during the dual-host adaptation of mosquito-borne flaviviruses.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The key amino acids of E protein involved in early flavivirus infection: viral entry.Virology journal · 2021Review
- Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients.Frontiers in immunology · 2021Article
48 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavivirus envelope proteins are synthesized as part of large polyproteins that are co- and posttranslationally cleaved into their individual chains. To investigate whether the interaction of neighboring proteins within the precursor protein is required to ensure proper maturation of the individual components, we have analyzed the folding of the flavivirus tick-borne encephalitis (TBE) virus envelope glycoproteins prM and E by using a recombinant plasmid expression system and virus-infected cells. When expressed in their polyprotein context, prM and E achieved their native folded structures with half-times of approximately 4 min for prM and about 15 min for E. They formed heterodimeric complexes within a few minutes after synthesis that were required for the final folding of E but not for that of prM. Heterodimers could also be formed in trans when these proteins were coexpressed from separate constructs. When expressed without prM, E could form disulfide bonds but did not express a specific conformational epitope and remained sensitive to reduction by dithiothreitol. This is consistent with a chaperone-like role for prM in the folding of E. PrM was able to achieve its native folded structure without coexpression of E, but signal sequence cleavage at the N terminus was delayed. Our results show that prM is an especially rapidly folding viral glycoprotein, that polyprotein cleavage and folding of the TBE virus envelope proteins occurs in a coordinated sequence of processing steps, and that proper and efficient maturation of prM and E can only be achieved by cosynthesis of these two proteins.
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