ArticleJournal of virology2002
Transduction of interphase cells by avian sarcoma virus.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 65 citations in OpenAlex.
- Engineered packaging cell line for the enhanced production of baboon-enveloped retroviral vectors.Molecular therapy. Nucleic acids · 2024Article
- The avian retroviral receptor Tva mediates the uptake of transcobalamin bound vitamin B12 (cobalamin).Journal of virology · 2021Article
- The Retrovirus Capsid Core.Sub-cellular biochemistry · 2018Review
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Retroviral DNA Integration.Chemical reviews · 2016Review
- Sites of retroviral DNA integration: From basic research to clinical applications.Critical reviews in biochemistry and molecular biology · 2016Review
- Retroviral DNA Transposition: Themes and Variations.Microbiology spectrum · 2014Article
- Article
- Nuclear localization signals in prototype foamy viral integrase for successive infection and replication in dividing cells.Molecules and cells · 2014Article
- Avian retroviral replication.Current opinion in virology · 2013Review
- The inside out of lentiviral vectors.Viruses · 2011Review
- Importin alpha3 interacts with HIV-1 integrase and contributes to HIV-1 nuclear import and replication.Journal of virology · 2010Article
- Modification of integration site preferences of an HIV-1-based vector by expression of a novel synthetic protein.Human gene therapy · 2010Article
- Ty3 nuclear entry is initiated by viruslike particle docking on GLFG nucleoporins.Journal of virology · 2009Article
- Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factors.Retrovirology · 2008Article
- The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing.Journal of virology · 2008Article
- A novel Flk1-TVA transgenic mouse model for gene delivery to angiogenic vasculature.Transgenic research · 2008Article
- The road to chromatin - nuclear entry of retroviruses.Nature reviews. Microbiology · 2007Review
- High-frequency epigenetic repression and silencing of retroviruses can be antagonized by histone deacetylase inhibitors and transcriptional activators, but uniform reactivation in cell clones is restricted by additional mechanisms.Journal of virology · 2007Article
- HIV infection of non-dividing cells: a divisive problem.Retrovirology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
It has been generally believed that oncoretroviruses are dependent on mitosis for efficient nuclear entry of viral DNA. We previously identified a nuclear localization signal in the integrase protein of an oncoretrovirus, avian sarcoma virus (ASV), suggesting an active import mechanism for the integrase-DNA complex (G. Kukolj, R. A. Katz, and A. M. Skalka, Gene 223:157-163, 1998). Here, we have evaluated the requirement for mitosis in nuclear import and integration of ASV DNA. Using a modified ASV encoding a murine leukemia virus amphotropic env gene and a green fluorescent protein (GFP) reporter gene, DNA nuclear import was measured in cell cycle-arrested avian (DF-1) as well as human (HeLa) and mouse cells. The results showed efficient accumulation of nuclear forms of ASV DNA in gamma-irradiation-arrested cells. Efficient transduction of a GFP reporter gene was also observed after infection of cells that were arrested with gamma-irradiation, mitomycin C, nocodazole, or aphidicolin, confirming that nuclear import and integration of ASV DNA can occur in the absence of mitosis. By monitoring GFP expression in individual cells, we also obtained evidence for nuclear import of viral DNA during interphase in cycling cells. Lastly, we observed that ASV can transduce postmitotic mouse neurons. These results support an active nuclear import mechanism for the oncoretrovirus ASV and suggest that this mechanism can operate in both nondividing and dividing cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.