ArticleRNA (New York, N.Y.)2002
Lead(II) as a probe for investigating RNA structure in vivo.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 80 citations in OpenAlex.
- Recent Advances in Chemical Probing Strategies for RNA Structure Determination In Vivo.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Effects of lipid membranes on RNA catalytic activity and stability.Biology of the cell · 2025Article
- Structural Analysis of the Hepatitis B Virus RNA Encapsidation Signal ε by Selective 2'-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE).Methods in molecular biology (Clifton, N.J.) · 2024Article
- Led-Seq: ligation-enhanced double-end sequence-based structure analysis of RNA.Nucleic acids research · 2023Article
- Incorporation of a FRET Pair into a Riboswitch RNA to Measure MgInternational journal of molecular sciences · 2022Article
- cyPhyRNA-seq: a genome-scale RNA-seq method to detect active self-cleaving ribozymes by capturing RNAs with 2',3'RNA biology · 2021Article
- Identification of host tRNAs preferentially recognized by the Plasmodium surface protein tRip.Nucleic acids research · 2021Article
- New RNA Structural Elements Identified in the Coding Region of the Coxsackie B3 Virus Genome.Viruses · 2020Article
- Lead-seq: transcriptome-wide structure probing in vivo using lead(II) ions.Nucleic acids research · 2020Article
- Unstructured 5'-tails act through ribosome standby to override inhibitory structure at ribosome binding sites.Nucleic acids research · 2018Article
- PATTERNA: transcriptome-wide search for functional RNA elements via structural data signatures.Genome biology · 2018Article
- The 5΄ UTR of the type I toxin ZorO can both inhibit and enhance translation.Nucleic acids research · 2017Article
- Comparative and integrative analysis of RNA structural profiling data: current practices and emerging questions.Quantitative biology (Beijing, China) · 2017Article
- Exploiting post-transcriptional regulation to probe RNA structures in vivo via fluorescence.Nucleic acids research · 2015Article
- Current perspectives on RNA secondary structure probing.Biochemical Society transactions · 2014Review
- Programmable folding of fusion RNA in vivo and in vitro driven by pRNA 3WJ motif of phi29 DNA packaging motor.Nucleic acids research · 2014Article
- How to find RNA thermometers.Frontiers in cellular and infection microbiology · 2014Review
- Identifying and characterizing Hfq-RNA interactions.Methods (San Diego, Calif.) · 2013Review
- Article
- Physical evidence supporting a ribosomal shunting mechanism of translation initiation for BACE1 mRNA.Translation (Austin, Tex.) · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this communication, we describe a simple and reliable method for RNA structure determination in vivo, using the divalent ion, lead(II), as a structural probe. Lead(II) is known to cleave RNA within single-stranded regions, loops, and bulges, whereas cleavages in double-stranded regions are weaker or absent. Because the ion easily entered bacterial cells, Escherichia coli cultures were treated by addition of 50-100 mM lead(II) acetate for 3-7 min, resulting in partial cleavage of RNA in vivo. Cleavage positions were mapped by reverse transcription analysis of total extracted RNA. Three RNAs were analyzed: tmRNA, CopT (the target of the antisense RNA CopA), and the leader region of the ompF mRNA. All three RNAs had previously been analyzed in vitro, and secondary structure models were available. The results presented here show that lead(II) cleavages in vivo yield detailed structural information for these RNAs, which was in good agreement with the models proposed based on in vitro work. These data illustrate the potential of lead(II) as a sequence-independent RNA structure probe for use in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.