ArticleJournal of biomolecular NMR2002
Towards high-resolution solid-state NMR on large uniformly 15N- and [13C,15N]-labeled membrane proteins in oriented lipid bilayers.
Article in Journal of biomolecular NMR, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Consensus structure of Pf1 filamentous bacteriophage from X-ray fibre diffraction and solid-state NMR.European biophysics journal : EBJ · 2011Article
- On the performance of spin diffusion NMR techniques in oriented solids: prospects for resonance assignments and distance measurements from separated local field experiments.The journal of physical chemistry. B · 2010Article
- The Role of Basic Amino Acids in the Molecular Recognition of Hydroxyapatite by Statherin using Solid State NMR.Surface science · 2010Article
- Sensitivity enhanced heteronuclear correlation spectroscopy in multidimensional solid-state NMR of oriented systems via chemical shift coherences.Journal of the American Chemical Society · 2010Article
- Resolution enhancement in solid-state NMR of oriented membrane proteins by anisotropic differential linebroadening.Journal of the American Chemical Society · 2008Article
- Solution NMR of signal peptidase, a membrane protein.Biochimica et biophysica acta · 2008Article
- Helix conformations in 7TM membrane proteins determined using oriented-sample solid-state NMR with multiple residue-specific 15N labeling.Biophysical journal · 2008Article
- Triple resonance experiments for aligned sample solid-state NMR of (13)C and (15)N labeled proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2007Article
- High-resolution NMR spectroscopy of a GPCR in aligned bicelles.Journal of the American Chemical Society · 2006Article
- Orientation of a beta-hairpin antimicrobial peptide in lipid bilayers from two-dimensional dipolar chemical-shift correlation NMR.Biophysical journal · 2006Article
- Reconstitution and alignment by a magnetic field of a beta-barrel membrane protein in bicelles.European biophysics journal : EBJ · 2006Article
- Can PISEMA experiments be used to extract structural parameters for mobile beta-barrels?Journal of biomolecular NMR · 2005Article
- Structure determination of membrane proteins by NMR spectroscopy.Chemical reviews · 2004Review
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Abstract
Based on exact numerical simulations, taking into account isotropic and conformation-dependent anisotropic nuclear spin interactions, we systematically analyse the prospects for high-resolution solid-state NMR on large isotope-labeled membrane proteins macroscopically oriented in phospholipid bilayers. Using the known X-ray structures of rhodopsin and porin as models for large membrane proteins with typical alpha-helical and beta-barrel structural motifs, the analysis considers all possible one- to six-dimensional spectra comprised of frequency dimensions with evolution under any combination of amide 1H, amide 15N, and carbonyl 13C chemical shifts as well as 1H-15N dipole-dipole couplings. Under consideration of typical nuclear spin interaction and experimental line-shape parameters, the analysis provides new insight into the resolution capability and orientation-dependent transfer efficiency of existing experiments as well as guidelines as to improved experimental approaches for the study of large uniformly 15N- and [13C,15N]-labeled membrane proteins. On basis of these results and numerical optimizations of coherence-transfer efficiencies, we propose several new high-resolution experiments for sequential protein backbone assignment and structure determination.
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