ArticleThe Biochemical journal2002
Diacylglycerol activates the influx of extracellular cations in T-lymphocytes independently of intracellular calcium-store depletion and possibly involving endogenous TRP6 gene products.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 83 citations in OpenAlex.
- Dantrolene normalizes heightened CaCell calcium · 2025Article
- New Insights on the Role of TRP Channels in Calcium Signalling and Immunomodulation: Review of Pathways and Implications for Clinical Practice.Clinical reviews in allergy & immunology · 2021Review
- The Antibody Receptor Fc Gamma Receptor IIIb Induces Calcium EntryFrontiers in immunology · 2021Article
- pH-Channeling in Cancer: How pH-Dependence of Cation Channels Shapes Cancer Pathophysiology.Cancers · 2020Review
- Mouse transient receptor potential channel type 6 selectively regulates agonist-induced platelet function.Biochemistry and biophysics reports · 2019Article
- Transient Receptor Potential (TRP) channels in T cells.Seminars in immunopathology · 2016Review
- Placing ion channels into a signaling network of T cells: from maturing thymocytes to healthy T lymphocytes or leukemic T lymphoblasts.BioMed research international · 2015Review
- Differential pathways for calcium influx activated by concanavalin A and CD3 stimulation in Jurkat T cells.Pflugers Archiv : European journal of physiology · 2012Article
- Altered protein kinase C regulation of pulmonary endothelial store- and receptor-operated Ca2+ entry after chronic hypoxia.The Journal of pharmacology and experimental therapeutics · 2010Article
- Regulatory activity of polyunsaturated fatty acids in T-cell signaling.Progress in lipid research · 2010Review
- Regulation of superoxide production in neutrophils: role of calcium influx.Journal of leukocyte biology · 2008Review
- Calcium-dependent transcription of cytokine genes in T lymphocytes.Pflugers Archiv : European journal of physiology · 2007Review
- Ion channel clustering enhances weak electric field detection by neutrophils: apparent roles of SKF96365-sensitive cation channels and myeloperoxidase trafficking in cellular responses.European biophysics journal : EBJ · 2005Article
- Physiological mechanisms of TRPC activation.Pflugers Archiv : European journal of physiology · 2005Review
- Determining the functional role of TRPC channels in primary cells.Pflugers Archiv : European journal of physiology · 2005Article
- Calcium-dependent activation of T-lymphocytes.Pflugers Archiv : European journal of physiology · 2005Review
- Amplification of receptor signalling by Ca2+ entry-mediated translocation and activation of PLCgamma2 in B lymphocytes.The EMBO journal · 2003Article
- TRPC6 is ubiquitously present in lymphatic tissues: A study using samples from body donors.Medicine internationalArticle
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Jurkat and human peripheral blood T-lymphocytes, 1-oleoyl-2-acetyl-sn-glycerol (OAG), a membrane-permeant analogue of diacylglycerol, activated the influx of Ca(2+), Ba(2+) and Sr(2+). OAG also caused plasma-membrane depolarization in Ca(2+)-free media that was recovered by the addition of bivalent cation, indicating the activation of Na(+) influx. OAG-induced cation influx was (i) mimicked by the natural dacylglycerol 1-stearoyl-2-arachidonyl-sn-glycerol, (ii) not blocked by inhibiting protein kinase C or in the absence of phospholipase C activity and (iii) blocked by La(3+) and Gd(3+). Differently from OAG, both thapsigargin and phytohaemagglutinin activated a potent influx of Ca(2+), but little influx of Ba(2+) and Sr(2+). Moreover, the influx of Ca(2+) activated by thapsigargin and that activated by OAG were additive. Furthermore, several drugs (i.e. econazole, SKF96365, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, 2-aminoethoxy diphenylborate and calyculin-A), while inhibiting the influx of Ca(2+) induced by both thapsigargin and phytohaemagglutinin, did not affect OAG-stimulated cation influx. Transient receptor potential (TRP) 3 and TRP6 proteins have been shown previously to be activated by diacylglycerol when expressed heterologously in animal cells [Hofmann, Obukhov, Schaefer, Harteneck, Gudermann and Schultz (1999) Nature (London) 397, 259-263]. In both Jurkat and peripheral blood T-lymphocytes, mRNA encoding TRP proteins 1, 3, 4 and 6 was detected by reverse transcriptase PCR, and the TRP6 protein was detected by Western blotting in a purified plasma-membrane fraction. We conclude that T-cells express a diacylglycerol-activated cation channel, unrelated to the channel involved in capacitative Ca(2+) entry, and associated with the expression of TRP6 protein.
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