ArticleJournal of clinical pathology2002
Validation of a simple, rapid, and economical technique for distinguishing type 1 and 2 fibres in fixed and frozen skeletal muscle.
Article in Journal of clinical pathology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Sex differences in human skeletal muscle fiber types and the influence of age, physical activity, and muscle group: A systematic review and meta-analysis.Physiological reports · 2025Pooled it
- Pre-Training Muscle Characteristics of Subjects Who Are Obese Determine How Well Exercise Training Will Improve Their Insulin Responsiveness.Journal of strength and conditioning research · 2017Trial
- Association between spontaneous neurogenic atrophy of the femoral muscle and islet cell tumors in aged F344 rats.Journal of toxicologic pathology · 2026Article
- Aging in the Skeletal Muscle Revealed by Molecular Immunohistochemical Imaging.International journal of molecular sciences · 2025Review
- The Na/K-ATPase α1/Src interaction regulates metabolic reserve and Western diet intolerance.Acta physiologica (Oxford, England) · 2021Article
- Fiber type composition of contiguous palmaris longus and abductor pollicis brevis muscles: Morphological evidence of a functional synergy.Journal of anatomy · 2021Article
- Skeletal Muscle Energetics and Mitochondrial Function Are Impaired Following 10 Days of Bed Rest in Older Adults.The journals of gerontology. Series A, Biological sciences and medical sciences · 2020Article
- Role of the Extracellular Matrix in Loss of Muscle Force With Age and Unloading Using Magnetic Resonance Imaging, Biochemical Analysis, and Computational Models.Frontiers in physiology · 2020Review
- Diffusion tensor imaging and diffusion modeling: Application to monitoring changes in the medial gastrocnemius in disuse atrophy induced by unilateral limb suspension.Journal of magnetic resonance imaging : JMRI · 2019Article
- Isoform-specific role of Na/K-ATPase α1 in skeletal muscle.American journal of physiology. Endocrinology and metabolism · 2018Article
- Neuromuscular Adaptations Following Training and Protein Supplementation in a Group of Trained Weightlifters.Sports (Basel, Switzerland) · 2018Article
- Muscle hypertrophy in prediabetic men after 16 wk of resistance training.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- Effects of Short-Term Free-Weight and Semiblock Periodization Resistance Training on Metabolic Syndrome.Journal of strength and conditioning research · 2016Article
- Myosin content of individual human muscle fibers isolated by laser capture microdissection.American journal of physiology. Cell physiology · 2016Article
- Insulin responsiveness in metabolic syndrome after eight weeks of cycle training.Medicine and science in sports and exercise · 2013Article
- Slow-twitch fiber proportion in skeletal muscle correlates with insulin responsiveness.The Journal of clinical endocrinology and metabolism · 2013Article
- Quantifiable diagnosis of muscular dystrophies and neurogenic atrophies through network analysis.BMC medicine · 2013Article
- Proliferative and non-proliferative lesions of the rat and mouse soft tissue, skeletal muscle and mesothelium.Journal of toxicologic pathology · 2013Review
- Impaired muscle AMPK activation in the metabolic syndrome may attenuate improved insulin action after exercise training.The Journal of clinical endocrinology and metabolism · 2011Article
- Myofiber ellipticity as an explanation for transverse asymmetry of skeletal muscle diffusion MRI in vivo signal.Annals of biomedical engineering · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo produce a method of distinguishing between type 1 and 2 skeletal muscle fibres that would be more economical and reproducible than the standard ATPase method and be applicable to both fixed and frozen tissue. Because the ATPase method has been accepted as the basis for fibre identification for the past 50 years, the new method should not give significantly different results.
methodsIsoforms of myosin correlate with isoforms of myofibrillar ATPase and an immunohistochemical (IHC) double labelling protocol was devised using monoclonal antibodies to fast and slow myosin. This required one tissue section rather than four. The results of the two methods were compared by means of morphometric analysis of skeletal muscle biopsies from 20 normal healthy volunteers.
resultsThere were no significant differences (p = 0.57) in the percentages of type 1 (46% using the IHC method v 48% using ATPase) or type 2 fibres (54% v 52%, respectively). The 2a and 2b subtypes were distinguished easily. Analysis of variance revealed that cross sectional area (mu m(2)), diameter (mu m), form factor, and density of fibre staining (a measure of substrate-enzyme or protein) were all similar. The method worked equally well on fixed material.
conclusionAn IHC method based on the fast and slow isoforms of myosin shows no significant differences in fibre type analysis from the standard ATPase method although it provides important advantages because it is applicable to fixed (including archival) material, is economical and reproducible, and yields a permanent preparation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.