ArticleImmunology2002
Age-related telomere length dynamics in peripheral blood mononuclear cells of healthy cynomolgus monkeys measured by Flow FISH.
Article in Immunology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Leukocyte telomere length and mitochondrial DNA copy number associate with endothelial function in aging-related cardiovascular disease.Frontiers in cardiovascular medicine · 2023Article
- Life Expectancy in Marine Mammals Is Unrelated to Telomere Length but Is Associated With Body Size.Frontiers in genetics · 2021Article
- Immunosenescent characteristics of T cells in young patients following haploidentical haematopoietic stem cell transplantation from parental donors.Clinical & translational immunology · 2020Article
- Telomere shortening rate predicts species life span.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Longer leukocyte telomere length in Costa Rica's Nicoya Peninsula: a population-based study.Experimental gerontology · 2013Article
- Empirical evaluation of humpback whale telomere length estimates; quality control and factors causing variability in the singleplex and multiplex qPCR methods.BMC genetics · 2012Article
- The effect of ageing on human lymphocyte subsets: comparison of males and females.Immunity & ageing : I & A · 2010Article
- Recovery from and consequences of severe iatrogenic lymphopenia (induced to treat autoimmune diseases).Clinical immunology (Orlando, Fla.) · 2004Article
- Passage-dependent changes in baboon endothelial cells--relevance to in vitro aging.DNA and cell biology · 2004Article
- Telomeres shorten more slowly in long-lived birds and mammals than in short-lived ones.Proceedings. Biological sciences · 2003Article
- Seasonal variation of peripheral blood leukocyte telomere length in Costa Rica: A population-based observational study.American journal of human biology : the official journal of the Human Biology CouncilObservational
- Coevolution of telomerase activity and body mass in mammals: from mice to beavers.Mechanisms of ageing and developmentReview
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomere length is a good biomarker to study the cellular senescence as well as aging of an organism, because it regulates the replicative capacity of vertebrate somatic cells. To demonstrate age-related telomere length dynamics in the peripheral blood mononuclear cells (PBMC) of the cynomolgus monkey, we introduced a novel method of measuring telomere length by fluorescence in situ hybridization with a Peptide Nucleic Acid (PNA) labelled probe and flow cytometry (Flow FISH). A highly significant correlation was observed between the intensity of telomere-specific fluorescence by Flow FISH and telomere length by Southern blot analysis (R = 0.923, n = 22). The intensity of telomere fluorescence in PBMC significantly decreased with age in 55 monkeys aged from 0 to 34 years and this decrease corresponded to the loss of 62.7 base pairs per year (R = - 0.52, P < 0.00004). We also analysed the expression of naive cell-associated markers, CD28, CD62L and CD45RA/CD62L in T lymphocytes of 47 cynomolgus monkeys. An age-related increase in the CD28- subset was observed in CD8+ T lymphocytes in monkeys less than 11 years old and in CD4+ T lymphocytes in monkeys over 23 years old, respectively. The percentage of CD62L+ subsets was significantly decreased with age in both CD4+ (R = - 0.55) and CD8+ T lymphocytes (R = - 0.73). From the comparison of telomere length among PBMC, CD62L+ and CD62L- T lymphocytes, it was clearly evident that loss of naive subsets results in the shortening of telomere length in vivo. These results show that this method can be applicable to studying the turnover and precursor-progeny of PBMC in cynomolgus monkeys as an animal model of aging.
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