ArticleProceedings of the National Academy of Sciences of the United States of America2002
Visualization of Ca2+ entry through single stretch-activated cation channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Lipid trapping slows ball-and-chain inactivation in a calcium-activated potassium channel.Nature communications · 2026Article
- Multiphysics and multiscale modeling of uterine contractions: integrating electrical dynamics and soft tissue deformation with fiber orientation.Medical & biological engineering & computing · 2024Article
- Article
- Mechanisms and physiological implications of cooperative gating of clustered ion channels.Physiological reviews · 2022Review
- Mechanotransduction in gastrointestinal smooth muscle cells: role of mechanosensitive ion channels.American journal of physiology. Gastrointestinal and liver physiology · 2021Review
- Contribution of TRPC Channels to Intracellular CaFrontiers in physiology · 2020Article
- Laminarin counteracts diet-induced obesity associated with glucagon-like peptide-1 secretion.Oncotarget · 2017Article
- Mechanosensitive Piezo Channels in the Gastrointestinal Tract.Current topics in membranes · 2017Review
- High-throughput optical sensing of nucleic acids in a nanopore array.Nature nanotechnology · 2015Article
- Single-molecule patch-clamp FRET microscopy studies of NMDA receptor ion channel dynamics in living cells: revealing the multiple conformational states associated with a channel at its electrical off state.Journal of the American Chemical Society · 2014Article
- Impact of heme and heme degradation products on vascular diameter in mouse visual cortex.Journal of the American Heart Association · 2014Article
- Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle.The Journal of general physiology · 2014Article
- Local membrane deformation and micro-injury lead to qualitatively different responses in osteoblasts.F1000Research · 2014Article
- Local regulation of L-type Ca²⁺ channel sparklets in arterial smooth muscle.Microcirculation (New York, N.Y. : 1994) · 2013Review
- Role of the transient receptor potential vanilloid type 1 receptor and stretch-activated ion channels in nitric oxide release from endothelial cells of the aorta and heart in rats.Experimental and clinical cardiology · 2012Article
- Spatial organization of RYRs and BK channels underlying the activation of STOCs by Ca(2+) sparks in airway myocytes.The Journal of general physiology · 2011Article
- From the stochasticity of molecular processes to the variability of synaptic transmission.Nature reviews. Neuroscience · 2011Review
- Natural inequalities: why some L-type Ca2+ channels work harder than others.The Journal of general physiology · 2010Article
- TRPC1 expression and distribution in rat hearts.European journal of histochemistry : EJH · 2009Article
- Molecular and biophysical mechanisms of Ca2+ sparklets in smooth muscle.Journal of molecular and cellular cardiology · 2009Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Stretch-activated channels (SACs) have been found in smooth muscle and are thought to be involved in myogenic responses. Although SACs have been shown to be Ca(2+) permeable when Ca(2+) is the only charge carrier, it has not been clearly demonstrated that significant Ca(2+) passes through SACs in physiological solutions. By imaging at high temporal and spatial resolution the single-channel Ca(2+) fluorescence transient (SCCaFT) arising from Ca(2+) entry through a single SAC opening, we provide direct evidence that significant Ca(2+) can indeed pass through SACs and increase the local [Ca(2+)]. Results were obtained under conditions where the only source of Ca(2+) was the physiological salt solution in the patch pipette containing 2 mM Ca(2+). Single smooth muscle cells were loaded with fluo-3 acetoxymethyl ester, and the fluorescence was recorded by using a wide-field digital imaging microscope while SAC currents were simultaneously recorded from cell-attached patches. Fluorescence increases at the cell-attached patch were clearly visualized before the simultaneous global Ca(2+) increase that occurred because of Ca(2+) influx through voltage-gated Ca(2+) channels when the membrane was depolarized by inward SAC current. From measurements of total fluorescence ("signal mass") we determined that about 18% of the SAC current is carried by Ca(2+) at membrane potentials more negative than the resting level. This would translate into at least a 0.35-pA unitary Ca(2+) current at the resting potential. Such Ca(2+) currents passing through SACs are sufficient to activate large-conductance Ca(2+)-activated K(+) channels and, as shown previously, to trigger Ca(2+) release from intracellular stores.
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