ArticleThe Biochemical journal2002
Uromodulin promoter directs high-level expression of biologically active human alpha1-antitrypsin into mouse urine.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 22 citations in OpenAlex.
- Expression systems and species used for transgenic animal bioreactors.BioMed research international · 2013Article
- Expanding the clinical indications for α(1)-antitrypsin therapy.Molecular medicine (Cambridge, Mass.) · 2012Review
- Expression of heme oxygenase-1 in thick ascending loop of henle attenuates angiotensin II-dependent hypertension.Journal of the American Society of Nephrology : JASN · 2012Article
- Transgenic mice and their impact on kidney research.Pflugers Archiv : European journal of physiology · 2009Review
- High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts.Transgenic research · 2009Article
- Production, purification, and characterization of human alpha1 proteinase inhibitor from Aspergillus niger.Biotechnology and bioengineering · 2009Article
- Animal transgenesis: state of the art and applications.Journal of applied genetics · 2007Review
- Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice.BMC biotechnology · 2005Article
- Renal tubule-specific expression and urinary secretion of human growth hormone: a kidney-based transgenic bioreactor growth.Transgenic research · 2003Article
- Kidney-specific activity of the bovine uromodulin promoter.Transgenic research · 2003Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently shown that the regulatory sequence of the uromodulin gene, containing the 3.7 kb promoter, exon 1 and a part of exon 2, provided for kidney-specific expression of the reporter lacZ gene in transgenic mice [Zbikowska, Soukhareva, Behnam, Chang, Drews, Lubon, Hammond and Soukharev (2002) Transgenic Res., in the press]. In the present study, we generated transgenic mice harbouring the regulatory sequence of the uromodulin gene to direct the expression of human alpha1-antitrypsin (alpha1AT) into urine. Of the 13 founder mice that tested positive by PCR, seven showed the presence of the human protein in their urine. The concentration of the recombinant human (rh) alpha1AT in the urine, estimated by using ELISA, ranged from 0.5 to 14 microg/ml in the F(0)-generation mice, and reached up to 65 microg/ml in the F1 generation. The transgenically produced rh alpha1AT was found to be N-glycosylated and biologically active. The N-terminal sequence analysis confirmed the identity of the human protein and revealed that the recombinant alpha1AT was correctly processed with the signal peptide cleaved off. Our results demonstrate for the first time that the uromodulin regulatory sequence provides a very attractive option for the potential large-scale production of functional therapeutic proteins in livestock.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.