ArticleThe Journal of general physiology2002
Effects of aldosterone on biosynthesis, traffic, and functional expression of epithelial sodium channels in A6 cells.
Article in The Journal of general physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 142 citations in OpenAlex.
- Kinetics of aldosterone-dependent ENaC trafficking in the kidney.The Journal of general physiology · 2025Article
- GRIN2B disease-associated mutations disrupt the function of BK channels and NMDA receptor signaling nanodomains.The Journal of general physiology · 2025Article
- Structural insights into subunit-dependent functional regulation in epithelial sodium channels.Structure (London, England : 1993) · 2025Article
- Case Report: Functional investigation of the γENaC G532S mutation presenting as mild PHA-1B3.Frontiers in medicine · 2025Article
- Glucocorticoids with or without fludrocortisone in septic shock: a narrative review from a biochemical and molecular perspective.British journal of anaesthesia · 2024Review
- Airway surface hyperviscosity and defective mucociliary transport by IL-17/TNF-α are corrected by β-adrenergic stimulus.JCI insight · 2022Article
- Gitelman syndrome with normocalciuria - a case report.BMC nephrology · 2022Article
- How SARS-CoV-2 might affect potassium balance via impairing epithelial sodium channels?Molecular biology reports · 2021Review
- Ubiquitination of renal ENaC subunits in vivo.American journal of physiology. Renal physiology · 2020Article
- Trichostatin A blocks aldosterone-induced NaBritish journal of pharmacology · 2019Article
- The epithelial NaPflugers Archiv : European journal of physiology · 2019Article
- The Epithelial Sodium Channel (αENaC) Is a Downstream Therapeutic Target of ASCL1 in Pulmonary Neuroendocrine Tumors.Translational oncology · 2018Article
- Inhibitors of the proteasome stimulate the epithelial sodium channel (ENaC) through SGK1 and mimic the effect of aldosterone.Pflugers Archiv : European journal of physiology · 2018Article
- Epithelial NaFrontiers in physiology · 2018Article
- High resolution measurement of membrane receptor endocytosis.Journal of biological methods · 2018Article
- Raman micro-spectroscopy as a viable tool to monitor and estimate the ionic transport in epithelial cells.Scientific reports · 2017Article
- Plasma membrane insertion of epithelial sodium channels occurs with dual kinetics.Pflugers Archiv : European journal of physiology · 2016Article
- Dexamethasone and insulin activate serum and glucocorticoid-inducible kinase 1 (SGK1) via different molecular mechanisms in cortical collecting duct cells.Physiological reports · 2016Article
- Regulation of ENaC trafficking in rat kidney.The Journal of general physiology · 2016Article
- The role of pendrin in blood pressure regulation.American journal of physiology. Renal physiology · 2016Review
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3 authors at 1 institution in 1 country.
Funding
Abstract
The collecting duct regulates Na(+) transport by adjusting the abundance/activity of epithelial Na(+) channels (ENaC). In this study we have investigated the synthesis, degradation, endocytosis, and activity of ENaC and the effects of aldosterone on these processes using endogenous channels expressed in the A6 cell line. Biochemical studies were performed with a newly raised set of specific antibodies against each of the three subunits of the amphibian ENaC. Our results indicate simultaneous transcription and translation of alpha, beta, and gamma subunits and enhancement of both processes by aldosterone: two- and fourfold increase, respectively. The biosynthesis of new channels can be followed by acquisition of endoglycosidase H-resistant oligosacharides in alpha and beta subunits and, in the case of alpha, by the appearance of a form resistant to reducing agents. The half-life of the total pool of subunits (t(1/2) 40-70 min) is longer than the fraction of channels in the apical membrane (t(1/2) 12-17 min). Aldosterone induces a fourfold increase in the abundance of the three subunits in the apical membrane without significant changes in the open probability, kinetics of single channels, or in the rate of degradation of ENaC subunits. Accordingly, the aldosterone response could be accounted by an increase in the abundance of apical channels due, at least in part, to de novo synthesis of subunits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.