ArticleJournal of clinical microbiology2002
Rapid identification of Penicillium marneffei by PCR-based detection of specific sequences on the rRNA gene.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- The delayed clearance of Talaromyces marneffei in blood culture may be associated with higher MIC of voriconazole after antifungal therapy among AIDS patients with talaromycosis.PLoS neglected tropical diseases · 2023Article
- Recurrent Talaromyces marneffei Infection Presenting with Intestinal Obstruction in a Patient with Systemic Lupus Erythematosus.Mycopathologia · 2020Article
- Extracellular Vesicles Derived FromFrontiers in microbiology · 2020Article
- Partial characteristics of hemolytic factors secreted from airborne Aspergillus and Penicillium, and an enhancement of hemolysis by Aspergillus micronesiensis CAMP-like factor via Staphylococcus aureus-sphingomyelinase.Journal of microbiology (Seoul, Korea) · 2019Article
- Characteristics and Prognosis of Talaromyces marneffei Infection in Non-HIV-Infected Children in Southern China.Mycopathologia · 2019Article
- Susceptibility profile of echinocandins, azoles and amphotericin B against yeast phase of Talaromyces marneffei isolated from HIV-infected patients in Guangdong, China.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Agrobacterium tumefaciens-mediated transformation: an efficient tool for targeted gene disruption in Talaromyces marneffei.World journal of microbiology & biotechnology · 2017Article
- Administration of Voriconazole in Disseminated Talaromyces (Penicillium) Marneffei Infection: A Retrospective Study.Mycopathologia · 2017Article
- Article
- Molecular Diagnosis of Pathogenic Sporothrix Species.PLoS neglected tropical diseases · 2015Article
- The pbrB gene encodes a laccase required for DHN-melanin synthesis in conidia of Talaromyces (Penicillium) marneffei.PloS one · 2015Article
- Functional analysis of atfA gene to stress response in pathogenic thermal dimorphic fungus Penicillium marneffei.PloS one · 2014Article
- Penicillium marneffei Infection in AIDS.Pathology research international · 2011Article
- Review
- Development and evaluation of loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Penicillium marneffei in archived tissue samples.FEMS immunology and medical microbiology · 2010Article
- Identification of Penicillium marneffei in Paraffin-Embedded Tissue Using Nested PCR.Mycopathologia · 2009Article
- Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects.Clinical microbiology reviews · 2006Review
- PCR assay for identification of histoplasma capsulatum based on the nucleotide sequence of the M antigen.Journal of clinical microbiology · 2003Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An emerging pathogenic dimorphic fungus, Penicillium marneffei, is one of the major causes of morbidity in patients with human immunodeficiency virus infection in Southeast Asia. A PCR-hybridization assay has been developed to identify this pathogen. This study describes the use of single and nested PCR methods for the rapid identification of P. marneffei. Two sets of oligonucleotide primers were derived from the sequence of 18S rRNA genes of P. marneffei. The outer primers (RRF1 and RRH1) were fungus specific. The inner primers (Pm1 and Pm2) were specific for P. marneffei and were used in nested or single PCR. The specific fragment of approximately 400-bp was amplified from both mold and yeast forms of 13 P. marneffei human isolates, 12 bamboo rat isolates, and 1 soil isolate, but not from other fungi, bacteria, and human DNA. The amplified products were analyzed by agarose gel electrophoresis followed by ethidium bromide staining. The sensitivities of the single PCR and nested PCR were 1.0 pg/microl and 1.8 fg/microl, respectively. The assay is useful for rapid identification of P. marneffei cultures. Very young culture of P. marneffei (2-day-old filamentous colony, 2 mm in diameter) could be performed by this assay. The species was identified within 7 h (single PCR) or 10 h (nested PCR), compared to 4 to 7 days for confirmation of dimorphism. The application of these PCR methods for early diagnosis of the disease needs to be studied further.
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