ArticleThe EMBO journal2002
Spi-B can functionally replace PU.1 in myeloid but not lymphoid development.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Regulation of lineage reprogramming by dynamic chromatin SUMOylation.Cellular and molecular life sciences : CMLS · 2026Article
- The ETS-family transcription factor PU.1 is a critical regulator of the inhibitory Fcγ receptor IIB expression in humans.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Single-cell chromatin profiling reveals genetic programs activating proregenerative states in nonmyocyte cells.Science advances · 2024Article
- Article
- Asymmetric Conservation within Pairs of Co-Occurred Motifs Mediates Weak Direct Binding of Transcription Factors in ChIP-Seq Data.International journal of molecular sciences · 2020Article
- Functional heterogeneity of alveolar macrophage population based on expression of CXCL2.Science immunology · 2020Article
- The ETS Family Transcription Factors Etv5 and PU.1 Function in Parallel To Promote Th9 Cell Development.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Nfkb1 activation by the E26 transformation-specific transcription factors PU.1 and Spi-B promotes Toll-like receptor-mediated splenic B cell proliferation.Molecular and cellular biology · 2015Article
- Retroviral infection of murine embryonic stem cell derived embryoid body cells for analysis of hematopoietic differentiation.Journal of visualized experiments : JoVE · 2014Article
- Plasmacytoid dendritic cells: development, functions, and role in atherosclerotic inflammation.Frontiers in physiology · 2014Review
- Dendritic cell development: a choose-your-own-adventure story.Advances in hematology · 2013Article
- Peyer's patch M cells derived from Lgr5(+) stem cells require SpiB and are induced by RankL in cultured "miniguts".Molecular and cellular biology · 2012Article
- Transcription factor Spi-B binds unique sequences present in the tandem repeat promoter/enhancer of JC virus and supports viral activity.The Journal of general virology · 2010Article
- Genome-wide analysis of ETS-family DNA-binding in vitro and in vivo.The EMBO journal · 2010Article
- Competition and collaboration: GATA-3, PU.1, and Notch signaling in early T-cell fate determination.Seminars in immunology · 2008Review
- Computational identification of the normal and perturbed genetic networks involved in myeloid differentiation and acute promyelocytic leukemia.Genome biology · 2008Article
- The Ets factor Spi-B is a direct critical target of the coactivator OBF-1.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Progression of regulatory gene expression states in fetal and adult pro-T-cell development.Immunological reviews · 2006Review
- Review
- Identification and characterization of a PU.1/Spi-B binding site in the bovine leukemia virus long terminal repeat.Oncogene · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Mature macrophages, neutrophils and lymphoid cells do not develop in PU.1(-/-) mice. In contrast, mice lacking the highly related protein Spi-B generate all hematopoietic lineages but display a B-cell receptor signaling defect. These distinct phenotypes could result from functional differences between PU.1 and Spi-B or their unique temporal and tissue-specific expression (PU.1: myeloid and B cells; Spi-B: B cells only). To address this question, we introduced the Spi-B cDNA into the murine PU.1 locus by homologous recombination. In the absence of PU.1, Spi-B rescued macrophage and granulocyte development when assayed by in vitro differentiation of embryonic stem cells. Adherent, CD11b(+)/F4/80(+) cells capable of phagocytosis were detected in PU.1(Spi-B/Spi-B) embryoid bodies, and myeloid colonies were present in hematopoietic progenitor assays. Despite its ability to rescue myeloid differentiation, Spi-B did not rescue lymphoid development in a RAG-2(-/-) complementation assay. These results demonstrate an important difference between PU.1 and Spi-B. Careful comparison of these Ets factors will delineate important functional domains of PU.1 involved in lymphocyte lineage commitment and/or maturation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.