ArticleThe EMBO journal2002
Export of autotransported proteins proceeds through an oligomeric ring shaped by C-terminal domains.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed.
- Construction and characterization of a nanopore derived from the transmembrane domain of a trimeric autotransporter adhesin.Frontiers in bioengineering and biotechnology · 2026Article
- The Secretion of Toxins and Other Exoproteins ofMicroorganisms · 2020Review
- Serine Protease Autotransporters of theMicroorganisms · 2019Review
- Escherichia coli surface display for the selection of nanobodies.Microbial biotechnology · 2017Review
- Analysis and Characterization of Proteins Associated with Outer Membrane Vesicles Secreted byFrontiers in microbiology · 2017Article
- A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity.Microbial cell factories · 2016Article
- Autotransporters: The Cellular Environment Reshapes a Folding Mechanism to Promote Protein Transport.The journal of physical chemistry letters · 2012Article
- The translocation domain in trimeric autotransporter adhesins is necessary and sufficient for trimerization and autotransportation.Journal of bacteriology · 2012Article
- Comparative analysis of the biochemical and functional properties of C-terminal domains of autotransporters.Journal of bacteriology · 2010Article
- Article
- Serine protease autotransporters of enterobacteriaceae (SPATEs): biogenesis and function.Toxins · 2010Review
- The expression of soluble and active recombinant Haemophilus influenzae IgA1 protease in E. coli.Journal of biomedicine & biotechnology · 2010Article
- Sab, a novel autotransporter of locus of enterocyte effacement-negative shiga-toxigenic Escherichia coli O113:H21, contributes to adherence and biofilm formation.Infection and immunity · 2009Article
- Active-site gating regulates substrate selectivity in a chymotrypsin-like serine protease the structure of haemophilus influenzae immunoglobulin A1 protease.Journal of molecular biology · 2009Article
- Contribution of trimeric autotransporter C-terminal domains of oligomeric coiled-coil adhesin (Oca) family members YadA, UspA1, EibA, and Hia to translocation of the YadA passenger domain and virulence of Yersinia enterocolitica.Journal of bacteriology · 2008Article
- Autotransporter structure reveals intra-barrel cleavage followed by conformational changes.Nature structural & molecular biology · 2007Article
- The autodisplay story, from discovery to biotechnical and biomedical applications.Microbiology and molecular biology reviews : MMBR · 2007Review
- Crystallization and preliminary X-ray diffraction analysis of P30, the transmembrane domain of pertactin, an autotransporter from Bordetella pertussis.Acta crystallographica. Section F, Structural biology and crystallization communications · 2007Article
- Relaxed cleavage specificity of an immunoglobulin A1 protease from Neisseria meningitidis.Infection and immunity · 2007Article
- Protease activity, secretion, cell entry, cytotoxicity, and cellular targets of secreted autotransporter toxin of uropathogenic Escherichia coli.Infection and immunity · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An investigation was made into the oligomerization, the ability to form pores and the secretion-related properties of the 45 kDa C-terminal domain of the IgA protease (C-IgAP) from Neisseria gonorrhoeae. This protease is the best studied example of the autotransporters (ATs), a large family of exoproteins from Gram-negative bacteria that includes numerous virulence factors from human pathogens. These proteins contain an N-terminal passenger domain that em bodies the secreted polypeptide, while the C-domain inserts into the outer membrane (OM) and trans locates the linked N-module into the extracellular medium. Here we report that purified C-IgAP forms an oligomeric complex of approximately 500 kDa with a ring-like structure containing a central cavity of approximately 2 nm diameter that is the conduit for the export of the N-domains. These data overcome the previous model for ATs, which postulated the passage of the N-module through the hydrophilic channel of the beta-barrel of each monomeric C-domain. Our results advocate a secretion mechanism not unlike other bacterial export systems, such as the secretins or fimbrial ushers, which rely on multimeric complexes assembled in the OM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.