ArticleJournal of bacteriology2002
The O-antigen gene cluster of Escherichia coli O55:H7 and identification of a new UDP-GlcNAc C4 epimerase gene.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 83 citations in OpenAlex.
- High diversity of Escherichia coli causing invasive disease in neonates in Malawi poses challenges for O-antigen based vaccine approach.Communications medicine · 2025Article
- Tracing the Evolutionary Pathways of Serogroup O78 Avian PathogenicAntibiotics (Basel, Switzerland) · 2023Article
- Global population structure, genomic diversity and carbohydrate fermentation characteristics of clonal complex 119 (CC119), an understudied Shiga toxin-producingMicrobial genomics · 2023Article
- Structure and genetics of Escherichia coli O antigens.FEMS microbiology reviews · 2020Review
- Capsular Polysaccharide Is a Receptor of aViruses · 2019Article
- Genomic analysis of carbon dioxide sequestering bacterium for exopolysaccharides production.Scientific reports · 2019Article
- TheFrontiers in cellular and infection microbiology · 2017Article
- Evidence for Contemporary Switching of the O-Antigen Gene Cluster between Shiga Toxin-ProducingFrontiers in microbiology · 2017Article
- Biochemical characterization of an α1,2-colitosyltransferase from Escherichia coli O55:H7.Glycobiology · 2016Article
- Biosynthesis of UDP-GlcNAc, UndPP-GlcNAc and UDP-GlcNAcA involves three easily distinguished 4-epimerase enzymes, Gne, Gnu and GnaB.PloS one · 2013Article
- Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water.International journal of microbiology · 2011Article
- Molecular and genetic analyses of the putative Proteus O antigen gene locus.Applied and environmental microbiology · 2010Article
- Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism.Molecular microbiology · 2010Article
- Genetic analysis for the lack of expression of the O157 antigen in an O Rough:H7 Escherichia coli strain.Applied and environmental microbiology · 2010Article
- A novel epimerase that converts GlcNAc-P-P-undecaprenol to GalNAc-P-P-undecaprenol in Escherichia coli O157.The Journal of biological chemistry · 2010Article
- Article
- A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence.Infection and immunity · 2008Article
- Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach.Applied and environmental microbiology · 2008Article
- Predicted functions and linkage specificities of the products of the Streptococcus pneumoniae capsular biosynthetic loci.Journal of bacteriology · 2007Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by comparison with known genes. Upstream of the normal O-antigen region there is a gne gene, which encodes a UDP-GlcNAc epimerase for converting UDP-GlcNAc to UDP-GalNAc and is essential for O55 antigen synthesis. The O55 gne product has only 20 and 26% identity to the gne genes of Pseudomonas aeruginosa and E. coli O113, respectively. We also found evidence for the O55 gene cluster's having evolved from another gene cluster by gain and loss of genes. Only three of the GDP-colitose pathway genes are in the usual location, the other two being separated, although nearby. It is thought that the E. coli O157:H7 clone evolved from the O55:H7 clone in part by transfer of the O157 gene cluster into an O55 lineage. Comparison of genes flanking the O-antigen gene clusters of the O55:H7 and O157:H7 clones revealed one recombination site within the galF gene and located the other between the hisG and amn genes. Genes outside the recombination sites are 99.6 to 100% identical in the two clones, while most genes thought to have transferred with the O157 gene cluster are 95 to 98% identical.
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