ArticleJournal of bacteriology2002
The membrane-associated protein-serine/threonine kinase from Sulfolobus solfataricus is a glycoprotein.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- 25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification.Journal of clinical biochemistry and nutrition · 2022Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Expanding the archaellum regulatory network - the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius.MicrobiologyOpen · 2017Article
- Protein phosphorylation and its role in archaeal signal transduction.FEMS microbiology reviews · 2016Review
- Protein Ser/Thr/Tyr phosphorylation in the Archaea.The Journal of biological chemistry · 2014Review
- Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.Microbiology and molecular biology reviews : MMBR · 2014Review
- Evidence for a novel Entamoeba histolytica lectin activity that recognises carbohydrates present on ovalbumin.International journal for parasitology · 2011Article
- Evidence that the xylanase activity from Sulfolobus solfataricus Oalpha is encoded by the endoglucanase precursor gene (sso1354) and characterization of the associated cellulase activity.Extremophiles : life under extreme conditions · 2008Article
- The alpha-L-fucosidase from Sulfolobus solfataricus.Extremophiles : life under extreme conditions · 2008Review
- A glycoproteome database of normal human liver tissue.Journal of cancer research and clinical oncology · 2007Article
- Posttranslational protein modification in Archaea.Microbiology and molecular biology reviews : MMBR · 2005Review
- A phosphoprotein from the archaeon Sulfolobus solfataricus with protein-serine/threonine kinase activity.Journal of bacteriology · 2004Article
- Deglycosylation of glycoproteins with trifluoromethanesulphonic acid: elucidation of molecular structure and function.The Biochemical journal · 2003Review
- Open reading frame sso2387 from the archaeon Sulfolobus solfataricus encodes a polypeptide with protein-serine kinase activity.Journal of bacteriology · 2003Article
- Archaeal protein kinases and protein phosphatases: insights from genomics and biochemistry.The Biochemical journal · 2003Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Treatment of a sodium dodecyl sulfate-polyacrylamide gel with periodic acid-Schiff (PAS) stain or blotting with Galanthus nivalis agglutinin revealed the presence of several glycosylated polypeptides in a partially purified detergent extract of the membrane fraction of Sulfolobus solfataricus. One of the glycoproteins comigrated with the membrane-associated protein-serine/threonine kinase from S. solfataricus, which had been radiolabeled by autophosphorylation with [(32)P]ATP in vitro. Treatment with a chemical deglycosylating agent, trifluoromethanesulfonic acid, abolished PAS staining and reduced the M(r) of the protein kinase from approximately 67,000 to approximately 62,000. Protein kinase activity also adhered to, and could be eluted from, agarose beads containing bound G. nivalis agglutinin. Glycosylation of the protein kinase implies that at least a portion of this integral membrane protein resides on the external surface of the cell membrane.
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