ArticleApplied and environmental microbiology2002
Cre-loxP recombination system for large genome rearrangements in Lactococcus lactis.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Metabolic engineering ofFood chemistry: X · 2026Article
- Establishment of a Cre-loxP System Based on a LeakyMicroorganisms · 2022Article
- Functional Identification of the Dextransucrase Gene ofInternational journal of molecular sciences · 2020Article
- Safety Aspects of Genetically Modified Lactic Acid Bacteria.Microorganisms · 2020Review
- Unrestrained markerless trait stacking inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Recombinase-mediated cassette exchange (RMCE) system for functional genomics studies in Mycoplasma mycoides.Biological procedures online · 2015Article
- A Tool for Multiple Targeted Genome Deletions that Is Precise, Scar-Free, and Suitable for Automation.PloS one · 2015Article
- Repetitive, marker-free, site-specific integration as a novel tool for multiple chromosomal integration of DNA.Applied and environmental microbiology · 2013Article
- Site-specific recombination strategies for engineering actinomycete genomes.Applied and environmental microbiology · 2012Article
- Lysine represses transcription of the Escherichia coli dapB gene by preventing its activation by the ArgP activator.Journal of bacteriology · 2008Article
- High-throughput identification and validation of in situ-expressed genes of Lactococcus lactis.Applied and environmental microbiology · 2008Article
- Markerless multiple-gene-deletion system for Streptococcus mutans.Applied and environmental microbiology · 2008Article
- Marker removal in staphylococci via Cre recombinase and different lox sites.Applied and environmental microbiology · 2008Article
- Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum.Applied and environmental microbiology · 2007Article
- Genome engineering in Bacillus anthracis using Cre recombinase.Infection and immunity · 2006Article
- New multiple-deletion method for the Corynebacterium glutamicum genome, using a mutant lox sequence.Applied and environmental microbiology · 2005Article
- Identification of Lactobacillus plantarum genes that are induced in the gastrointestinal tract of mice.Journal of bacteriology · 2004Article
- Use of the alr gene as a food-grade selection marker in lactic acid bacteria.Applied and environmental microbiology · 2002Article
- Modern Approaches to the Genome Editing of Antibiotic Biosynthetic Clusters in Actinomycetes.Acta naturaeArticle
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used a new genetic strategy based on the Cre-loxP recombination system to generate large chromosomal rearrangements in Lactococcus lactis. Two loxP sites were sequentially integrated in inverse order into the chromosome either at random locations by transposition or at fixed points by homologous recombination. The recombination between the two chromosomal loxP sites was highly efficient (approximately 1 x 10(-1)/cell) when the Cre recombinase was provided in trans, and parental- or inverted-type chromosomal structures were isolated after removal of the Cre recombinase. The usefulness of this approach was demonstrated by creating three large inversions of 500, 1,115, and 1,160 kb in size that modified the lactococcal genome organization to different extents. The Cre-loxP recombination system described can potentially be used for other gram-positive bacteria without further modification.
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