ArticleApplied and environmental microbiology2002
Genomic subtraction to identify and characterize sequences of Shiga toxin-producing Escherichia coli O91:H21.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Enterohemorrhagic Escherichia coli pathogenesis: role of Long polar fimbriae in Peyer's patches interactions.Scientific reports · 2017Article
- In vitro adhesion properties of Shiga toxin-producing Escherichia coli isolated from cattle, food, and humans.Frontiers in microbiology · 2015Article
- Interactions with M cells and macrophages as key steps in the pathogenesis of enterohemorrhagic Escherichia coli infections.PloS one · 2011Article
- Effect of probe characteristics on the subtractive hybridization efficiency of human genomic DNA.BMC research notes · 2010Article
- Next-generation sequencing and metagenomic analysis: a universal diagnostic tool in plant virology.Molecular plant pathology · 2009Article
- Presence and characterization of a mosaic genomic island which distinguishes sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- from E. coli O157:H7.Applied and environmental microbiology · 2005Article
- A glimpse into the expanded genome content of Vibrio cholerae through identification of genes present in environmental strains.Journal of bacteriology · 2005Article
- Real-time PCR assay for a unique chromosomal sequence of Bacillus anthracis.Journal of clinical microbiology · 2004Article
- Distribution of putative adhesins in different seropathotypes of Shiga toxin-producing Escherichia coli.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify Shiga toxin-producing Escherichia coli genes associated with severe human disease, a genomic subtraction technique was used with hemolytic-uremic syndrome-associated O91:H21 strain CH014 and O6:H10 bovine strains. The method was adapted to the Shiga toxin-producing E. coli genome: three rounds of subtraction were used to isolate DNA fragments specific to strain CH014. The fragments were characterized by genetic support analysis, sequencing, and hybridization to the genome of a collection of Shiga toxin-producing E. coli strains. A total of 42 fragments were found, 19 of which correspond to previously identified unique DNA sequences in the enterohemorrhagic E. coli EDL933 reference strain, including 7 fragments corresponding to prophage sequences and others encoding candidate virulence factors, such a SepA homolog protein and a fimbrial usher protein. In addition, the subtraction procedure yielded plasmid-related sequences from Shigella flexneri and enteropathogenic and Shiga toxin-producing E. coli virulence plasmids. We found that lateral gene transfer is extensive in strain CH014, and we discuss the role of genomic mobile elements, especially bacteriophages, in the evolution and possible transfer of virulence determinants.
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