ArticleGenetics2002
A complex array of DNA-binding proteins required for pairing-sensitive silencing by a polycomb group response element from the Drosophila engrailed gene.
Article in Genetics, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 95 citations in OpenAlex.
- Article
- The impact of cell states on heterochromatin dynamics.The Biochemical journal · 2024Review
- Article
- Polycomb protein binding and looping in the ON transcriptional state.Science advances · 2024Article
- Polycomb protein binding and looping mediated by Polycomb Response Elements in the ON transcriptional state.bioRxiv : the preprint server for biology · 2023Article
- Polycomb Recruiters Inside and Outside of the Repressed Domains.International journal of molecular sciences · 2023Review
- Crol contributes to PRE-mediated repression and Polycomb group proteins recruitment in Drosophila.Nucleic acids research · 2023Article
- Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogaster.G3 (Bethesda, Md.) · 2021Article
- Boundaries potentiate polycomb response element-mediated silencing.BMC biology · 2021Article
- GAGA factor: a multifunctional pioneering chromatin protein.Cellular and molecular life sciences : CMLS · 2021Review
- Mapping of functional elements of the Fab-6 boundary involved in the regulation of the Abd-B hox gene in Drosophila melanogaster.Scientific reports · 2021Article
- A theoretical model of Polycomb/Trithorax action unites stable epigenetic memory and dynamic regulation.Nature communications · 2020Article
- Dynamic Competition of Polycomb and Trithorax in Transcriptional Programming.Annual review of biochemistry · 2020Review
- Core Components of the Nuclear Pore Bind Distinct States of Chromatin and Contribute to Polycomb Repression.Molecular cell · 2020Article
- DNA sequence models of genome-wide Drosophila melanogaster Polycomb binding sites improve generalization to independent Polycomb Response Elements.Nucleic acids research · 2019Article
- Sex combs reduced (Scr) regulatory region of Drosophila revisited.Molecular genetics and genomics : MGG · 2017Article
- Polycomb and Trithorax Group Genes inGenetics · 2017Review
- Dual functionality ofGenes & development · 2017Article
- Formation of a Polycomb-Domain in the Absence of Strong Polycomb Response Elements.PLoS genetics · 2016Article
- Heterochromatin-Associated Proteins HP1a and Piwi Collaborate to Maintain the Association of Achiasmate Homologs in Drosophila Oocytes.Genetics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Regulatory DNA from the Drosophila gene engrailed causes silencing of a linked reporter gene (mini-white) in transgenic Drosophila. This silencing is strengthened in flies homozygous for the transgene and has been called "pairing-sensitive silencing." The pairing-sensitive silencing activities of a large fragment (2.6 kb) and a small subfragment (181 bp) were explored. Since pairing-sensitive silencing is often associated with Polycomb group response elements (PREs), we tested the activities of each of these engrailed fragments in a construct designed to detect PRE activity in embryos. Both fragments were found to behave as PREs in a bxd-Ubx-lacZ reporter construct, while the larger fragment showed additional silencing capabilities. Using the mini-white reporter gene, a 139-bp minimal pairing-sensitive element (PSE) was defined. DNA mobility-shift assays using Drosophila nuclear extracts suggested that there are eight protein-binding sites within this 139-bp element. Mutational analysis showed that at least five of these sites are important for pairing-sensitive silencing. One of the required sites is for the Polycomb group protein Pleiohomeotic and another is GAGAG, a sequence bound by the proteins GAGA factor and Pipsqueak. The identity of the other proteins is unknown. These data suggest a surprising degree of complexity in the DNA-binding proteins required for PSE function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.