ArticleNucleic acids research2002
High throughput measurement of duplex, triplex and quadruplex melting curves using molecular beacons and a LightCycler.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Highly Potent Fluorogenic Ligands for Triplex DNA: 5-Substituted 2-(Naphthalen-2-yl)-4H-Chromen-4-Ones.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Rational design of an acridine-derived click chemistry-based artificial metallo-nuclease.RSC chemical biology · 2026Article
- Fluorous-Directed Clamping Stabilizes Triple-Helical DNA.ACS omega · 2026Article
- Recognition of non-standard base pairs by triplex-forming oligonucleotides containing an expanded genetic alphabet.Nature communications · 2026Article
- Recognition of Non-standard Base Pairs by Triplex-Forming Oligonucleotides Containing an Expanded Genetic Alphabet.Research square · 2026Article
- High-throughput measurement and prediction of the i-motif DNA stability landscape.Nucleic acids research · 2026Article
- Recent progress in probing small molecule interactions with DNA.Biophysical reviews · 2025Review
- 5-Substituted Flavones-Another Class of Potent Triplex DNA-Specific Ligands as Antigene Enhancers.Molecules (Basel, Switzerland) · 2024Article
- Structural elucidation of HIV-1 G-quadruplexes in a cellular environment and their ligand binding using responsiveChemical science · 2024Article
- A simplified and easy-to-use HIP HOP assay provides insights into chalcone antifungal mechanisms of action.FEBS letters · 2022Article
- Fluorophore-mediated Photooxidation of the Guanine Heterocycle.Journal of physical organic chemistry · 2022Article
- Drug discovery of small molecules targeting the higher-order hTERT promoter G-quadruplex.PloS one · 2022Article
- Triplex-forming properties and enzymatic incorporation of a base-modified nucleotide capable of duplex DNA recognition at neutral pH.Nucleic acids research · 2021Article
- Article
- Melting temperature measurement and mesoscopic evaluation of single, double and triple DNA mismatches.Chemical science · 2020Article
- A 'light-up' intercalator displacement assay for detection of triplex DNA stabilizers.Chemical communications (Cambridge, England) · 2020Article
- Article
- Modulation of DNA structure formation using small molecules.Biochimica et biophysica acta. Molecular cell research · 2019Review
- A tunable assay for modulators of genome-destabilizing DNA structures.Nucleic acids research · 2019Article
- Probing G-quadruplex topologies and recognition concurrently in real time and 3D using a dual-app nucleoside probe.Nucleic acids research · 2019Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have used oligonucleotides containing molecular beacons to determine melting profiles for intramolecular DNA duplexes, triplexes and quadruplexes (tetraplexes). The synthetic oligonucleotides used in these studies contain a fluorophore (fluorescein) and quencher (methyl red) attached either to deoxyribose or to the 5 position of dU. In the folded DNA structures the fluorophore and quencher are in close proximity and the fluorescence is quenched. When the structures melt, the fluorophore and quencher are separated and there is a large increase in fluorescence. These experiments were performed in a Roche LightCycler; this requires small amounts of material (typically 4 pmol oligonucleotide) and can perform 32 melting profiles in parallel. We have used this technique to compare the stability of triplexes containing different base analogues and to confirm the selectivity of a triplex-binding ligand for triplex, rather than duplex, DNA. We have also compared the melting of inter- and intramolecular quadruplexes.
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