ArticleNucleic acids research2002
Two self-splicing group I introns in the ribonucleotide reductase large subunit gene of Staphylococcus aureus phage Twort.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 53 citations in OpenAlex.
- The Biology of Nucleus-Forming Jumbo Phages.Annual review of genetics · 2025Review
- Development of advanced control material for reverse transcription-mediated bacterial nucleic acid amplification tests.Journal of clinical microbiology · 2024Article
- Genome Study of a Novel Virulent Phage vB_SspS_KASIA and Mu-like Prophages ofInternational journal of molecular sciences · 2021Article
- Isolation and Characterization of a Novel Phage SaGU1 that Infects Staphylococcus aureus Clinical Isolates from Patients with Atopic Dermatitis.Current microbiology · 2021Article
- Isolation and Characterization ofAntibiotics (Basel, Switzerland) · 2020Article
- Characterization of vB_SauM-fRuSau02, a Twort-Like Bacteriophage Isolated from a Therapeutic Phage Cocktail.Viruses · 2017Article
- Three novel Pseudomonas phages isolated from composting provide insights into the evolution and diversity of tailed phages.BMC genomics · 2017Article
- Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains.PloS one · 2017Article
- Article
- Single-Cell (Meta-)Genomics of a Dimorphic Candidatus Thiomargarita nelsonii Reveals Genomic Plasticity.Frontiers in microbiology · 2016Article
- Bacterial group I introns: mobile RNA catalysts.Mobile DNA · 2014Article
- Phages of non-dairy lactococci: isolation and characterization of ΦL47, a phage infecting the grass isolate Lactococcus lactis ssp. cremoris DPC6860.Frontiers in microbiology · 2014Article
- Genome analysis of the staphylococcal temperate phage DW2 and functional studies on the endolysin and tail hydrolase.Bacteriophage · 2014Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Romulus and Remus, two phage isolates representing a distinct clade within the Twortlikevirus genus, display suitable properties for phage therapy applications.Journal of virology · 2013Article
- A bioinformatic analysis of ribonucleotide reductase genes in phage genomes and metagenomes.BMC evolutionary biology · 2013Article
- Bacillus subtilis hlpB encodes a conserved stand-alone HNH nuclease-like protein that is essential for viability unless the hlpB deletion is accompanied by the deletion of genes encoding the AddAB DNA repair complex.Journal of bacteriology · 2012Article
- Complete genome sequence of Staphylococcus aureus bacteriophage GH15.Journal of virology · 2012Article
- Article
- A homing endonuclease and the 50-nt ribosomal bypass sequence of phage T4 constitute a mobile DNA cassette.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have recently described three group I introns inserted into a single gene, orf142, of the staphylococcal bacteriophage Twort and suggested the presence of at least two additional self-splicing introns in this phage genome. Here we report that two previously uncharacterized introns, 429 and 1087 nt in length, interrupt the Twort gene coding for the large subunit of ribonucleotide reductase (nrdE). Reverse transcription-polymerase chain reaction (RT-PCR) of RNA isolated from Staphylococcus aureus after phage infection indicates that the introns are removed from the primary transcript in vivo. Both nrdE introns show sequence similarity to the Twort orf142 introns I2 and I3, suggesting either a common origin of these introns or shuffling of intron structural elements. Intron 2 encodes a DNA endonuclease, I-TwoI, with similarity to homing endonucleases of the HNH family. Like I-HmuI and I-HmuII, intron-encoded HNH endonucleases in Bacillus subtilis phages SPO1 and SP82, I-TwoI nicks only one strand of its DNA recognition sequence. However, whereas I-HmuI and I-HmuII cleave the template strand in exon 2, I-TwoI cleaves the coding strand in exon 1. In each case, the 3' OH created on the cut strand is positioned to prime DNA synthesis towards the intron, suggesting that this reaction contributes to the mechanism of intron homing. Both nrdE introns are inserted in highly conserved regions of the ribonucleotide reductase gene, next to codons for functionally important residues.
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