ArticleMolecular and cellular biology2002
Alteration of large-scale chromatin structure by estrogen receptor.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 97 citations in OpenAlex.
- The potential of epigenetic therapy to target the 3D epigenome in endocrine-resistant breast cancer.Nature structural & molecular biology · 2024Article
- Dual role of deubiquitinating enzyme USP19 regulates mitotic progression and tumorigenesis by stabilizing survivin.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- Histone deacetylase inhibitors suppress transdifferentiation of gonadotrophs to prolactin cells and proliferation of prolactin cells induced by diethylstilbestrol in male mouse pituitary.Histochemistry and cell biology · 2019Article
- Myc-driven chromatin accessibility regulates Cdc45 assembly into CMG helicases.Communications biology · 2019Article
- Signaling by Steroid Hormones in the 3D Nuclear Space.International journal of molecular sciences · 2018Review
- Glucocorticoid Receptor Binding Induces Rapid and Prolonged Large-Scale Chromatin Decompaction at Multiple Target Loci.Cell reports · 2017Article
- The 5-Hydroxymethylcytosine (5hmC) Reader UHRF2 Is Required for Normal Levels of 5hmC in Mouse Adult Brain and Spatial Learning and Memory.The Journal of biological chemistry · 2017Article
- GR and ER Coactivation Alters the Expression of Differentiation Genes and Associates with Improved ER+ Breast Cancer Outcome.Molecular cancer research : MCR · 2016Article
- Chromatin interaction analysis reveals changes in small chromosome and telomere clustering between epithelial and breast cancer cells.Genome biology · 2015Article
- Estrogen-induced chromatin decondensation and nuclear re-organization linked to regional epigenetic regulation in breast cancer.Genome biology · 2015Article
- Distinct structural transitions of chromatin topological domains correlate with coordinated hormone-induced gene regulation.Genes & development · 2014Article
- Estrogen induces global reorganization of chromatin structure in human breast cancer cells.PloS one · 2014Article
- A novel histone H4 arginine 3 methylation-sensitive histone H4 binding activity and transcriptional regulatory function for signal recognition particle subunits SRP68 and SRP72.The Journal of biological chemistry · 2012Article
- DDB2 promotes chromatin decondensation at UV-induced DNA damage.The Journal of cell biology · 2012Article
- Chromatin loops, gene positioning, and gene expression.Frontiers in genetics · 2012Article
- S phase-dependent interaction with DNMT1 dictates the role of UHRF1 but not UHRF2 in DNA methylation maintenance.Cell research · 2011Article
- Two-step binding of transcription factors causes sequential chromatin structural changes at the activated IL-2 promoter.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Chromatin unfolding by Cdt1 regulates MCM loading via opposing functions of HBO1 and HDAC11-geminin.Cell cycle (Georgetown, Tex.) · 2010Article
- Activation of estrogen-responsive genes does not require their nuclear co-localization.PLoS genetics · 2010Article
- The spatial dynamics of tissue-specific promoters during C. elegans development.Genes & development · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The estrogen receptor (ER), a member of the nuclear hormone receptor superfamily important in human physiology and disease, recruits coactivators which modify local chromatin structure. Here we describe effects of ER on large-scale chromatin structure as visualized in live cells. We targeted ER to gene-amplified chromosome arms containing large numbers of lac operator sites either directly, through a lac repressor-ER fusion protein (lac rep-ER), or indirectly, by fusing lac repressor with the ER interaction domain of the coactivator steroid receptor coactivator 1. Significant decondensation of large-scale chromatin structure, comparable to that produced by the approximately 150-fold-stronger viral protein 16 (VP16) transcriptional activator, was produced by ER in the absence of estradiol using both approaches. Addition of estradiol induced a partial reversal of this unfolding by green fluorescent protein-lac rep-ER but not by wild-type ER recruited by a lac repressor-SRC570-780 fusion protein. The chromatin decondensation activity did not require transcriptional activation by ER nor did it require ligand-induced coactivator interactions, and unfolding did not correlate with histone hyperacetylation. Ligand-induced coactivator interactions with helix 12 of ER were necessary for the partial refolding of chromatin in response to estradiol using the lac rep-ER tethering system. This work demonstrates that when tethered or recruited to DNA, ER possesses a novel large-scale chromatin unfolding activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.