ArticleThe Journal of cell biology2002
Dynamics of ligand-induced, Rac1-dependent anchoring of cadherins to the actin cytoskeleton.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 120 citations in OpenAlex.
- Phosphorylation of Focal Adhesion Kinase at Y925: Role in Glia-Dependent and Independent Migration through Regulating Cofilin and N-Cadherin.Molecular neurobiology · 2022Article
- Intercellular adhesion molecule-1 augments myoblast adhesion and fusion through homophilic trans-interactions.Scientific reports · 2017Article
- A phenomenological model of cell-cell adhesion mediated by cadherins.Journal of mathematical biology · 2017Article
- Integration of Cadherin Adhesion and Cytoskeleton atCold Spring Harbor perspectives in biology · 2017Review
- Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation.Molecular biology of the cell · 2017Article
- The mechanotransduction machinery at work at adherens junctions.Integrative biology : quantitative biosciences from nano to macro · 2015Review
- The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity.The Journal of cell biology · 2015Article
- Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines.Molecular biology of the cell · 2015Article
- Cadherins as regulators of neuronal polarity.Cell adhesion & migration · 2015Review
- The interdependence of the Rho GTPases and apicobasal cell polarity.Small GTPases · 2014Review
- Covalent and density-controlled surface immobilization of E-cadherin for adhesion force spectroscopy.PloS one · 2014Article
- CD148 tyrosine phosphatase promotes cadherin cell adhesion.PloS one · 2014Article
- FSGS3/CD2AP is a barbed-end capping protein that stabilizes actin and strengthens adherens junctions.The Journal of cell biology · 2013Article
- Binding to F-actin guides cadherin cluster assembly, stability, and movement.The Journal of cell biology · 2013Article
- α-Catenin and vinculin cooperate to promote high E-cadherin-based adhesion strength.The Journal of biological chemistry · 2013Article
- A bigger picture: classical cadherins and the dynamic actin cytoskeleton.Nature reviews. Molecular cell biology · 2012Review
- Activation of ezrin/radixin/moesin mediates attractive growth cone guidance through regulation of growth cone actin and adhesion receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Ajuba is required for Rac activation and maintenance of E-cadherin adhesion.The Journal of cell biology · 2011Article
- Negative feedback from integrins to cadherins: a micromechanical study.Biophysical journal · 2011Article
- Tissue organization by cadherin adhesion molecules: dynamic molecular and cellular mechanisms of morphogenetic regulation.Physiological reviews · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cadherin receptors are key morphoregulatory molecules during development. To dissect their mode of action, we developed an approach based on the use of myogenic C2 cells and beads coated with an Ncad-Fc ligand, allowing us to mimic cadherin-mediated adhesion. We used optical tweezers and video microscopy to investigate the dynamics of N-cadherin anchoring within the very first seconds of bead-cell contact. The analysis of the bead movement by single-particle tracking indicated that N-cadherin molecules were freely diffusive in the first few seconds after bead binding. The beads rapidly became diffusion-restricted and underwent an oriented rearward movement as a result of N-cadherin anchoring to the actin cytoskeleton. The kinetics of anchoring were dependent on ligand density, suggesting that it was an inducible process triggered by active cadherin recruitment. This anchoring was inhibited by the dominant negative form of Rac1, but not that of Cdc42. The Rac1 mutant had no effect on cell contact formation or cadherin-catenin complex recruitment, but did inhibit actin recruitment. Our results suggest that cadherin anchoring to the actin cytoskeleton is an adhesion-triggered, Rac1-regulated process enabling the transduction of mechanical forces across the cell membrane; they uncover novel aspects of the action of cadherins in cell sorting, cell migration, and growth cone navigation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.