ArticleYeast (Chichester, England)2002
Isolation and mapping of self-assembling protein domains encoded by the Saccharomyces cerevisiae genome using lambda repressor fusions.
Article in Yeast (Chichester, England), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Single-cell imaging of protein dynamics of paralogs reveals sources of gene retention.iScience · 2025Article
- Sec7 regulatory domains scaffold autoinhibited and active conformations.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Phosphoproteomic Analysis of Rat Neutrophils Shows the Effect of Intestinal Ischemia/Reperfusion and Preconditioning on Kinases and Phosphatases.International journal of molecular sciences · 2020Article
- The HUS box is required for allosteric regulation of the Sec7 Arf-GEF.The Journal of biological chemistry · 2018Article
- The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.eLife · 2016Article
- The Sec7 Arf-GEF is recruited to the trans-Golgi network by positive feedback.Developmental cell · 2012Article
- Genomes and characterization of phages Bcep22 and BcepIL02, founders of a novel phage type in Burkholderia cenocepacia.Journal of bacteriology · 2011Article
- The oligomerization of CynR in Escherichia coli.Protein science : a publication of the Protein Society · 2009Article
- The oligomerization of OxyR in Escherichia coli.Protein science : a publication of the Protein Society · 2009Article
- Similar modes of interaction enable Trailer Hitch and EDC3 to associate with DCP1 and Me31B in distinct protein complexes.Molecular and cellular biology · 2008Article
- Crystal structure of human Edc3 and its functional implications.Molecular and cellular biology · 2008Article
- Statistical analysis of interface similarity in crystals of homologous proteins.Journal of molecular biology · 2008Article
- Combining guilt-by-association and guilt-by-profiling to predict Saccharomyces cerevisiae gene function.Genome biology · 2008Article
- Edc3p and a glutamine/asparagine-rich domain of Lsm4p function in processing body assembly in Saccharomyces cerevisiae.The Journal of cell biology · 2007Article
- Genome-wide prediction and characterization of interactions between transcription factors in Saccharomyces cerevisiae.Nucleic acids research · 2006Article
- Divergence and mosaicism among virulent soil phages of the Burkholderia cepacia complex.Journal of bacteriology · 2006Article
- Identification and mapping of self-assembling protein domains encoded by the Escherichia coli K-12 genome by use of lambda repressor fusions.Journal of bacteriology · 2004Article
- On the number of protein-protein interactions in the yeast proteome.Nucleic acids research · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Understanding how proteins are able to form stable complexes is of fundamental interest from the perspective of protein structure and function. Here we show that lambda repressor fusions can be used to identify and characterize homotypic interaction domains encoded by the genome of Saccharomyces cerevisiae, using a selection for polypeptides that can drive the assembly of the DNA binding domain of bacteriophage lambda repressor. Three high complexity libraries were constructed by cloning random fragments of S. cerevisiae DNA as lambda repressor fusions. Repressor fusions encoding homotypic interactions were recovered, identifying oligomerization units in 35 yeast proteins. Seventeen of these interaction domains have not been previously reported, while the other 18 represent homotypic interactions that have been characterized at varying levels of detail. The novel interactions include several predicted coiled-coils as well as domains of unknown structure. With the availability of genomic sequences it should be possible to apply this approach, which provides information about protein-protein interactions that is complementary to that obtained from yeast two-hybrid screens, on a genome-wide scale in yeast or other organisms where large-scale protein-protein interaction data is not available.
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