ArticleProtein science : a publication of the Protein Society2002
Self-assembly of the hydrophobin SC3 proceeds via two structural intermediates.
Article in Protein science : a publication of the Protein Society, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Solution NMR reveals micelle-stabilized α-helical segments flanking the RIPK1 RHIM amyloid core.Biochemical and biophysical research communications · 2026Article
- AlphaFold modeling uncovers global structural features of class I and class II fungal hydrophobins.Protein science : a publication of the Protein Society · 2025Article
- Identification of Candidate Ice Nucleation Activity (INA) Genes inJournal of fungi (Basel, Switzerland) · 2022Article
- Versatile biomimetic medical device surface: hydrophobin coated, nitric oxide-releasing polymer for antimicrobial and hemocompatible applications.Biomaterials science · 2019Article
- Hydrophobins: multifunctional biosurfactants for interface engineering.Journal of biological engineering · 2019Review
- Cerato-populin and cerato-platanin, two non-catalytic proteins from phytopathogenic fungi, interact with hydrophobic inanimate surfaces and leaves.Molecular biotechnology · 2013Article
- Crystallization and preliminary X-ray diffraction of the surfactant protein Lv-ranaspumin from the frog Leptodactylus vastus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Hydrophobins--unique fungal proteins.PLoS pathogens · 2012Article
- Absence of repellents in Ustilago maydis induces genes encoding small secreted proteins.Antonie van Leeuwenhoek · 2011Article
- Expression and purification of a functionally active class I fungal hydrophobin from the entomopathogenic fungus Beauveria bassiana in E. coli.Journal of industrial microbiology & biotechnology · 2011Article
- Creating Surface Properties Using a Palette of Hydrophobins.Materials (Basel, Switzerland) · 2010Review
- Recombinantly produced hydrophobins from fungal analogues as highly surface-active performance proteins.European biophysics journal : EBJ · 2010Article
- Assembly of the fungal SC3 hydrophobin into functional amyloid fibrils depends on its concentration and is promoted by cell wall polysaccharides.The Journal of biological chemistry · 2009Article
- Structural basis for rodlet assembly in fungal hydrophobins.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The SC3 hydrophobin self-assembles into a membrane with distinct mass transfer properties.Biophysical journal · 2005Article
- Probing the self-assembly and the accompanying structural changes of hydrophobin SC3 on a hydrophobic surface by mass spectrometry.Biophysical journal · 2004Article
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9 authors.
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Abstract
Hydrophobins self assemble into amphipathic films at hydrophobic-hydrophilic interfaces. These proteins are involved in a broad range of processes in fungal development. We have studied the conformational changes that accompany the self-assembly of the hydrophobin SC3 with polarization-modulation infrared reflection absorption spectroscopy, attenuated total reflection Fourier transform infrared spectroscopy, and circular dichroism, and related them to changes in morphology as observed by electron microcopy. Three states of SC3 have been spectroscopically identified previously as follows: the monomeric state, the alpha-helical state that is formed upon binding to a hydrophobic solid, and the beta-sheet state, which is formed at the air-water interface. Here, we show that the formation of the beta-sheet state of SC3 proceeds via two intermediates. The first intermediate has an infrared spectrum indistinguishable from that of the alpha-helical state of SC3. The second intermediate is rich in beta-sheet structure and has a featureless appearance under the electron microscope. The end state has the same secondary structure, but is characterized by the familiar 10-nm-wide rodlets.
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