ArticleJournal of virology2002
Positive and negative modulation of human immunodeficiency virus type 1 Rev function by cis and trans regulators of viral RNA splicing.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Host cell factors in HIV replication: meta-analysis of genome-wide studies.PLoS pathogens · 2009Pooled it
- HIV-1: To Splice or Not to Splice, That Is the Question.Viruses · 2021Review
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- Embryonic expression of endogenous retroviral RNAs in somatic tissues adjacent to the Oikopleura germline.Nucleic acids research · 2015Article
- Article
- A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1.Retrovirology · 2014Article
- Reevaluation of the reliability and usefulness of the somatic homologous recombination reporter lines.The Plant cell · 2012Review
- Article
- Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA.Retrovirology · 2008Article
- Splicing regulation: from a parts list of regulatory elements to an integrated splicing code.RNA (New York, N.Y.) · 2008Review
- hnRNP E1 and E2 have distinct roles in modulating HIV-1 gene expression.Retrovirology · 2007Article
- Use of modified U1 snRNAs to inhibit HIV-1 replication.Nucleic acids research · 2007Article
- The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus.Structure (London, England : 1993) · 2006Article
- The retrovirus RNA trafficking granule: from birth to maturity.Retrovirology · 2006Review
- A novel function for Sam68: enhancement of HIV-1 RNA 3' end processing.RNA (New York, N.Y.) · 2004Article
- PSF acts through the human immunodeficiency virus type 1 mRNA instability elements to regulate virus expression.Molecular and cellular biology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the entire complement of human immunodeficiency virus type 1 (HIV-1) viral proteins depends on the competing activities of viral RNA splicing and export into the cytoplasm by Rev. To investigate the possibility that modulation of viral RNA metabolism may alter Rev function, we analyzed the impact of multiple SR proteins on both processes. While overexpression of several of the SR factors altered splicing of HIV-1 env mRNA, they had disparate effects on Rev function that varied with the cell line used. Subsequent examination of exon splicing enhancer (ESE) and/or silencer (ESS) deletions suggests that the effects of the SR proteins on Rev function are not mediated through interaction with these elements. However, analysis of the deletions did indicate that the ESE and/or ESS does have significant effects on Rev function, with deletion of the ESS augmenting the magnitude of the response to Rev and deletion of the ESE significantly reducing it. In situ hybridization and reverse transcription-PCR indicated that the loss of Rev response upon deletion of the ESE was due to a failure of Rev to induce transport of the unspliced RNA into the cytoplasm. Together, the data indicate that cellular splicing factors and viral regulatory elements can have significant stimulatory and inhibitory effects on Rev function, raising the possibility that cells can be rendered permissive or nonpermissive for virus replication by modulation of splicing activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.