ArticleJournal of virology2002
Infectious virions produced from a human papillomavirus type 18/16 genomic DNA chimera.
Article in Journal of virology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- A Comparative Study on Delivery of Externally Attached DNA by Papillomavirus VLPs and Pseudoviruses.Vaccines · 2021Article
- Superinfection Exclusion between Two High-Risk Human Papillomavirus Types during a Coinfection.Journal of virology · 2018Article
- Recent advances in preclinical model systems for papillomaviruses.Virus research · 2017Review
- The CXCL12/CXCR4 Signaling Pathway: A New Susceptibility Factor in Human Papillomavirus Pathogenesis.PLoS pathogens · 2016Article
- Three-dimensional cell culture models for investigating human viruses.Virologica Sinica · 2016Review
- Article
- Review
- Phylogenetic considerations in designing a broadly protective multimeric L2 vaccine.Journal of virology · 2013Article
- Human papillomavirus type 18 chimeras containing the L2/L1 capsid genes from evolutionarily diverse papillomavirus types generate infectious virus.Virus research · 2011Article
- Papillomavirus capsid proteins mutually impact structure.Virology · 2011Article
- Article
- Downregulation of Cdc2/CDK1 kinase activity induces the synthesis of noninfectious human papillomavirus type 31b virions in organotypic tissues exposed to benzo[a]pyrene.Journal of virology · 2010Article
- Robust production and passaging of infectious HPV in squamous epithelium of primary human keratinocytes.Genes & development · 2009Article
- The cigarette smoke carcinogen benzo[a]pyrene enhances human papillomavirus synthesis.Journal of virology · 2008Article
- Human papillomavirus type 31b infection of human keratinocytes does not require heparan sulfate.Journal of virology · 2005Article
- Genetic analysis of the human papillomavirus type 31 differentiation-dependent late promoter.Journal of virology · 2005Article
- Propagation of infectious human papillomavirus type 16 by using an adenovirus and Cre/LoxP mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Saccharomyces cerevisiae is permissive for replication of bovine papillomavirus type 1.Journal of virology · 2002Article
- Human papillomavirus type 31b infection of human keratinocytes and the onset of early transcription.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The organotypic raft culture system has allowed the study of the differentiation-dependent aspects of the human papillomavirus (HPV) life cycle. However, genetic strategies to more completely understand the HPV life cycle are limited. The generation of chimeric viruses has been a useful tool in other virus systems to analyze infection and replication. To investigate the specificity of the interaction of nonstructural genes of one HPV type with the structural genes of another HPV type, we have replaced the L2 and L1 open reading frames (ORFs) of HPV type 18 (HPV18) with the L2 and L1 ORFs of HPV type 16 (HPV16). The resulting HPV18/16 chimeric construct was introduced into primary keratinocytes, where it was stably maintained episomally at a range of 50 to 100 copies of HPV genomic DNA, similar to that typically found in HPV-infected cells in vivo. The integrity of the HPV18/16 genomic DNA chimera was demonstrated. Upon differentiation in raft cultures, late viral functions, including viral DNA amplification, capsid gene expression, and virion morphogenesis, occurred. Chimeric HPV18/16 virions were purified from the raft cultures and were capable of infecting keratinocytes in vitro. Additionally, infection was specifically neutralized with human HPV16 virus-like particle (VLP)-specific antiserum and not with human HPV18 VLP-specific antiserum. Our data demonstrate that the nonstructural genes of HPV18 functionally interact with the structural genes of HPV16, allowing the complete HPV life cycle to occur. We believe that this is the first report of the propagation of chimeric HPV by normal life cycle pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.