ArticleEMBO reports2002
Identification of genes by hybrid ribozymes that couple cleavage activity with the unwinding activity of an endogenous RNA helicase.
Article in EMBO reports, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 59 citations in OpenAlex.
- Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome.Virology journal · 2009Article
- 3'-Poly(A) tail enhances siRNA activity against exogenous reporter genes in MCF-7 cells.Journal of RNAi and gene silencing : an international journal of RNA and gene targeting research · 2006Article
- Identification of genes by hybrid ribozymes that couple cleavage activity with the unwinding activity of an endogenous RNA helicase.EMBO reports · 2006Article
- Identification of cellular cofactors for human immunodeficiency virus replication via a ribozyme-based genomics approach.Journal of virology · 2004Article
- An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease.The EMBO journal · 2004Article
- Effects on RNAi of the tight structure, sequence and position of the targeted region.Nucleic acids research · 2004Article
- Cell migration and metastasis as targets of small RNA-based molecular genetic analyses.Journal of muscle research and cell motility · 2004Review
- Ribozyme-based gene-inactivation systems require a fine comprehension of their substrate specificities; the case of delta ribozyme.Current medicinal chemistry · 2003Article
- Expressing functional siRNAs in mammalian cells using convergent transcription.BMC biotechnology · 2003Article
- Targeting mortalin using conventional and RNA-helicase-coupled hammerhead ribozymes.EMBO reports · 2003Article
- Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells.Nucleic acids research · 2003Article
- Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A functional gene discovery in the Fas-mediated pathway to apoptosis by analysis of transiently expressed randomized hybrid-ribozyme libraries.Nucleic acids research · 2002Article
Corrections and comments
- Retraction · 2006-07-01Concerns/Issues about Data · Ethical Violations by Author ·
- Retracted
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Novel ribozymes that couple the cleavage activity of hammerhead ribozymes with the unwinding activity of RNA helicase eIF4AI were constructed. This leads to extremely efficient cleavage of the target mRNA, regardless of the secondary structure of the RNA, and eliminates one of the major problems: many target sites on the RNA were previously inaccessible to cleavage due to secondary and/or tertiary structure formation. Moreover, libraries of hybrid ribozymes with randomized binding arms were introduced into cells. This procedure made it possible to readily identify the relevant genes associated with phenotype. Specifically, four genes known to be in the Fas-mediated apoptosis pathway were identified along with additional genes. This application of a randomized library of hybrid ribozymes represents a simple, powerful method for the identification of genes associated with specific phenotypes in the post-genome era.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.