ArticleEMBO reports2002
Spontaneous insertion and partitioning of alkaline phosphatase into model lipid rafts.
Article in EMBO reports, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 140 citations in OpenAlex.
- Interaction of Tau construct K18 with model lipid membranes.Nanoscale advances · 2021Article
- Dynamic Continuum of Nanoscale Peptide Assemblies Facilitates Endocytosis and Endosomal Escape.Nano letters · 2021Article
- A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study.Methods in molecular biology (Clifton, N.J.) · 2021Article
- γ-Secretase Partitioning into Lipid Bilayers Remodels Membrane Microdomains after Direct Insertion.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Biomimetic Models to Investigate Membrane Biophysics Affecting Lipid-Protein Interaction.Frontiers in bioengineering and biotechnology · 2020Review
- Effects of GPI-anchored TNAP on the dynamic structure of model membranes.Physical chemistry chemical physics : PCCP · 2015Article
- Lipid raft association restricts CD44-ezrin interaction and promotion of breast cancer cell migration.The American journal of pathology · 2012Article
- Thermodynamic properties and characterization of proteoliposomes rich in microdomains carrying alkaline phosphatase.Biophysical chemistry · 2011Article
- Dynamic modulation of the glycosphingolipid content in supported lipid bilayers by glycolipid transfer protein.Biophysical journal · 2010Article
- Material properties of lipid microdomains: force-volume imaging study of the effect of cholesterol on lipid microdomain rigidity.Biophysical journal · 2010Article
- Proteoliposomes as matrix vesicles' biomimetics to study the initiation of skeletal mineralization.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2010Review
- Dynamic partitioning of a glycosyl-phosphatidylinositol-anchored protein in glycosphingolipid-rich microdomains imaged by single-quantum dot tracking.Traffic (Copenhagen, Denmark) · 2009Article
- Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM.Pflugers Archiv : European journal of physiology · 2008Review
- Atomic force microscopy imaging of Bacillus thuringiensis Cry1 toxins interacting with insect midgut apical membranes.The Journal of membrane biology · 2008Article
- Transition from nanodomains to microdomains induced by exposure of lipid monolayers to air.Biophysical journal · 2007Article
- High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer.Biophysical journal · 2006Article
- Effects of ceramide on liquid-ordered domains investigated by simultaneous AFM and FCS.Biophysical journal · 2006Article
- Correlated fluorescence-atomic force microscopy of membrane domains: structure of fluorescence probes determines lipid localization.Biophysical journal · 2006Article
- Epidermal growth factor receptors are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: a shotgun lipidomics study.The Journal of biological chemistry · 2005Article
- Following the formation of supported lipid bilayers on mica: a study combining AFM, QCM-D, and ellipsometry.Biophysical journal · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several cell surface eukaryotic proteins have a glycosylphosphatidylinositol (GPI) modification at the C-terminal end that serves as an anchor to the plasma membrane and could be responsible for the presence of GPI proteins in rafts, a type of functionally important membrane microdomain enriched in sphingolipids and cholesterol. In order to understand better how GPI proteins partition into rafts, the insertion of the GPI-anchored alkaline phosphatase (AP) was studied in real-time using atomic force microscopy. Supported phospholipid bilayers made of a mixture of sphingomyelin-dioleoylphosphatidylcholine containing cholesterol (Chl+) or not (Chl-) were used to mimic the fluid-ordered lipid phase separation in biological membranes. Spontaneous insertion of AP through its GPI anchor was observed inside both Chl+ and Chl- lipid ordered domains, but AP insertion was markedly increased by the presence of cholesterol.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.