ArticleThe Biochemical journal2002
Identification of Ca2+-dependent binding partners for the neuronal calcium sensor protein neurocalcin delta: interaction with actin, clathrin and tubulin.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed.
- Machine Learning-Based Classification of Transcriptome Signatures of Non-Ulcerative Bladder Pain Syndrome.International journal of molecular sciences · 2024Article
- Machine Learning-based Classification of transcriptome Signatures of non-ulcerative Bladder Pain Syndrome.bioRxiv : the preprint server for biology · 2024Article
- Multiple time points of transcriptome analysis revealed altered genes involved in maintaining hibernation in the hypothalamus ofFrontiers in neuroscience · 2024Article
- Long-TermInternational journal of molecular sciences · 2023Article
- Mitochondrial Dysfunction in Spinal Muscular Atrophy.International journal of molecular sciences · 2022Review
- Gene Expression Profiling in the Striatum ofBiomolecules & therapeutics · 2021Article
- Calcium-Dependent Translocation of S100B Is Facilitated by Neurocalcin Delta.Molecules (Basel, Switzerland) · 2021Article
- Mouse mutant phenotyping at scale reveals novel genes controlling bone mineral density.PLoS genetics · 2020Article
- Membrane Binding of Neuronal Calcium Sensor-1: Highly Specific Interaction with Phosphatidylinositol-3-Phosphate.Biomolecules · 2020Article
- Clinicopathological and prognostic significance of NCALD protein expression in lung adenocarcinoma.Translational cancer research · 2019Article
- Calcium Sensors in Neuronal Function and Dysfunction.Cold Spring Harbor perspectives in biology · 2019Review
- Neurocalcin Delta Knockout Impairs Adult Neurogenesis Whereas Half Reduction Is Not Pathological.Frontiers in molecular neuroscience · 2019Article
- Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.American journal of human genetics · 2017Article
- Atrial natriuretic factor receptor guanylate cyclase, ANF-RGC, transduces two independent signals, ANF and Ca(2+).Frontiers in molecular neuroscience · 2014Review
- Ca(2+)-modulated ROS-GC1 transduction system in testes and its presence in the spermatogenic cells.Frontiers in molecular neuroscience · 2014Article
- Regulatory subunit myristoylation antagonizes calcineurin phosphatase activation in yeast.The Journal of biological chemistry · 2012Article
- Calcineurin homologous protein: a multifunctional Ca2+-binding protein family.American journal of physiology. Renal physiology · 2012Review
- The visinin-like proteins VILIP-1 and VILIP-3 in Alzheimer's disease-old wine in new bottles.Frontiers in molecular neuroscience · 2012Article
- Article
- Antithetical modes of and the Ca(2+) sensors targeting in ANF-RGC and ROS-GC1 membrane guanylate cyclases.Frontiers in molecular neuroscience · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The neuronal calcium sensors are a family of EF-hand-containing Ca(2+)-binding proteins expressed predominantly in retinal photoreceptors and neurons. One of the family members is neurocalcin delta, the function of which is unknown. As an approach to elucidating the protein interactions made by neurocalcin delta, we have identified brain cytosolic proteins that bind to neurocalcin delta in a Ca(2+)-dependent manner. We used immobilized recombinant myristoylated neurocalcin delta combined with protein identification using MS. We demonstrate a specific interaction with clathrin heavy chain, alpha- and beta-tubulin, and actin. These interactions were dependent upon myristoylation of neurocalcin delta indicating that the N-terminal myristoyl group may be important for protein-protein interactions in addition to membrane association. Direct binding of neurocalcin delta to clathrin, tubulin and actin was confirmed using an overlay assay. These interactions were also demonstrated for endogenous neurocalcin delta by co-immunoprecipitation from rat brain cytosol. When expressed in HeLa cells, neurocalcin delta was cytosolic at resting Ca(2+) levels but translocated to membranes, including a perinuclear compartment (trans-Golgi network) where it co-localized with clathrin, following Ca(2+) elevation. These data suggest the possibility that neurocalcin delta functions in the control of clathrin-coated vesicle traffic.
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Registered trials
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