ArticleProceedings of the National Academy of Sciences of the United States of America2002
Developmental specificity of recruitment of TBP to the TATA box of the human gamma-globin gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
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- Elucidating the importance and regulation of key enhancers for human MEIS1 expression.Leukemia · 2022Article
- Genomic environments scale the activities of diverse core promoters.Genome research · 2022Article
- Characterization and Expression of the Lucina pectinata Oxygen and Sulfide Binding Hemoglobin Genes.PloS one · 2016Article
- Expression of different functional isoforms in haematopoiesis.International journal of hematology · 2014Review
- Regulation of eukaryotic gene expression by the untranslated gene regions and other non-coding elements.Cellular and molecular life sciences : CMLS · 2012Review
- Article
- Identification of E74-like factor 1 (ELF1) as a transcriptional regulator of the Hox cofactor MEIS1.Experimental hematology · 2010Article
- A T9G mutation in the prototype TATA-box TCACTATATATAG determines nucleosome formation and synergy with upstream activator sequences in plant promoters.Plant physiology · 2009Article
- The higher structure of chromatin in the LCR of the beta-globin locus changes during development.Journal of molecular biology · 2009Article
- Noncanonical TATA sequence in the UL44 late promoter of human cytomegalovirus is required for the accumulation of late viral transcripts.Journal of virology · 2008Article
- Architecture of the sporulation-specific Cdc14 promoter from the oomycete Phytophthora infestans.Eukaryotic cell · 2007Article
- Real-time monitoring of functional interactions between upstream and core promoter sequences in living cells of sea urchin embryos.Nucleic acids research · 2007Article
- The TATA-box sequence in the basal promoter contributes to determining light-dependent gene expression in plants.Plant physiology · 2006Article
- Autonomous silencing as well as competition controls gamma-globin gene expression during development.Molecular and cellular biology · 2006Article
- Transcriptional potential of the gamma-globin gene is dependent on the CACCC box in a developmental stage-specific manner.Nucleic acids research · 2006Article
- Synergistic and additive properties of the beta-globin locus control region (LCR) revealed by 5'HS3 deletion mutations: implication for LCR chromatin architecture.Molecular and cellular biology · 2005Article
- Differences of globin transgene expression in stably transfected cell lines and transgenic mice.Blood · 2005Article
- The minimal promoter plays a major role in silencing of the galago gamma-globin gene in adult erythropoiesis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Developmentally specific role of the CCAAT box in regulation of human gamma-globin gene expression.The Journal of biological chemistry · 2004Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is unclear whether the core promoter is involved in developmental regulation. To address this question, we mutated the TATA box of the human gamma-globin gene, produced transgenic mice, and examined the effect of the mutation during the course of mouse development. In our test system, the gamma-globin gene is expressed at similar levels in the embryonic and adult erythroid cells. The TATA box mutation dramatically reduced expression of the gamma-globin gene in the adult but not in embryonic erythroid cells. In addition, the disruption of the gamma TATA box significantly reduced the recruitment of TATA box-binding protein (TBP) in the adult cells, but not in embryonic cells, suggesting that the recruitment of TBP to the gamma gene promoter is developmentally specific. Similarly, the recruitment of transcription factor II B and RNA polymerase II to the gamma promoter was affected in the adult but not in embryonic cells. The distinct effects of the TATA mutation in the embryonic and adult developmental stages suggest that the basal transcription apparatus can be recruited to a core promoter in a developmental stage-dependent manner. The TATA mutation resulted in a shift of transcription initiation site 6 bp or longer upstream to the cap site both in the embryonic and adult erythrocytes. We conclude that the TATA box determines the initiation site but not the efficiency of transcription of the gamma-globin gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.