ArticleProceedings of the National Academy of Sciences of the United States of America2002
Modulation of protein translation by Nck-1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- Regulation of trophoblast fusion dysfunction in preeclampsia by the NCK1/EIF3D/PPARG axis.Biological research · 2026Article
- Long noncoding RNAs are substrates for cytoplasmic capping enzyme.FEBS letters · 2023Article
- Nck adaptors at a glance.Journal of cell science · 2021Article
- NCK1-AS1 promotes the progression of lung squamous cell carcinoma through transcriptionally upregulating NCK1 via interacting with MYC.Cancer biology & therapy · 2021Article
- Sinner or Saint?: Nck Adaptor Proteins in Vascular Biology.Frontiers in cell and developmental biology · 2021Review
- Peptide-based sequestration of the adaptor protein Nck1 in pancreatic β cells enhances insulin biogenesis and protects against diabetogenic stresses.The Journal of biological chemistry · 2018Article
- Deletion of eIF2β lysine stretches creates a dominant negative that affects the translation and proliferation in human cell line: A tool for arresting the cell growth.Cancer biology & therapy · 2017Article
- mTORC1 and CK2 coordinate ternary and eIF4F complex assembly.Nature communications · 2016Article
- Nck1 depletion induces activation of the PI3K/Akt pathway by attenuating PTP1B protein expression.Cell communication and signaling : CCS · 2014Article
- The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme.PLoS biology · 2014Article
- Dissecting the roles of tyrosines 490 and 785 of TrkA protein in the induction of downstream protein phosphorylation using chimeric receptors.The Journal of biological chemistry · 2013Article
- Transmembrane receptor DCC associates with protein synthesis machinery and regulates translation.Cell · 2010Article
- The adapter protein Nck: role of individual SH3 and SH2 binding modules for protein interactions in T lymphocytes.Protein science : a publication of the Protein Society · 2010Article
- Nck adapter proteins: functional versatility in T cells.Cell communication and signaling : CCS · 2009Article
- Evaluation of in vitro cytotoxicity and paracellular permeability of intact monolayers with mouse embryonic stem cells.Toxicology in vitro : an international journal published in association with BIBRA · 2008Article
- Proteomic analysis of tyrosine phosphorylation during human liver transplantation.Proteome science · 2007Article
- Nephrin ectodomain engagement results in Src kinase activation, nephrin phosphorylation, Nck recruitment, and actin polymerization.The Journal of clinical investigation · 2006Article
- The adaptor protein Nck interacts with Fas ligand: Guiding the death factor to the cytotoxic immunological synapse.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The N-terminal domain of the human eIF2beta subunit and the CK2 phosphorylation sites are required for its function.The Biochemical journal · 2006Article
- Tyrosine phosphorylation acts as a molecular switch to full-scale activation of the eIF2alpha RNA-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals, Nck represented by two genes, is a 47-kDa SH2/SH3 domain-containing protein lacking intrinsic enzymatic function. Here, we reported that the first and the third SH3 domains of Nck-1 interact with the C-terminal region of the beta subunit of the eukaryotic initiation factor 2 (eIF2 beta). Binding of eIF2 beta was specific to the SH3 domains of Nck-1, and in vivo, the interaction Nck/eIF2 beta was demonstrated by reciprocal coimmunoprecipitations. In addition, Nck was detected in a molecular complex with eIF2 beta in an enriched ribosomal fraction, whereas no other SH2/SH3 domain-containing adapters were found. Cell fractionation studies demonstrated that the presence of Nck in purified ribosomal fractions was enhanced after insulin stimulation, suggesting that growth factors dynamically regulate translocation of Nck to ribosomes. In HEK293 cells, we observed that transient overexpression of Nck-1 significantly enhanced Cap-dependent and -independent protein translation. This effect of Nck-1 required the integrity of its first and third SH3 domains originally found to interact with eIF2 beta. Finally, in vitro, Nck-1 also increased protein translation, revealing a direct role for Nck-1 in this process. Our study demonstrates that in addition to mediate receptor tyrosine kinase signaling, Nck-1 modulates protein translation potentially through its direct interaction with an intrinsic component of the protein translation machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.