ArticleProceedings of the National Academy of Sciences of the United States of America2002
A minimal system allowing tubulation with molecular motors pulling on giant liposomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
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Who cites it
95 citing papers in PubMed, 304 citations in OpenAlex.
- Article
- Photoisomerizing molecules in biological membranes.Nature reviews. Chemistry · 2026Review
- Topology and kinetic pathways of colloidosome assembly and disassembly.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploration and analytical techniques for membrane curvature-sensing proteins in bacteria.Journal of bacteriology · 2025Review
- Migrasome formation is initiated preferentially in tubular junctions by membrane tension.Biophysical journal · 2025Article
- LRK-1/LRRK2 and AP-3 regulate trafficking of synaptic vesicle precursors through active zone protein SYD-2/Liprin-α.PLoS genetics · 2024Article
- Membrane Tubulation with a Biomembrane Force Probe.Membranes · 2023Article
- Formation of protein-mediated bilayer tubes is governed by a snapthrough transition.Soft matter · 2023Article
- Organelle Membrane Extensions in Mammalian Cells.Biology · 2023Review
- Interaction of Hyaluronan Acid with Some Proteins in Aqueous Solution as Studied by NMR.Membranes · 2023Article
- Review
- Choreographing the motor-driven endosomal dance.Journal of cell science · 2023Review
- Article
- Intelligent fluorescence image analysis of giant unilamellar vesicles using convolutional neural network.BMC bioinformatics · 2022Article
- An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Mechanics and Thermodynamics of Tubule Formation in Biological Membranes.The Journal of membrane biology · 2021Review
- Pattern formation and polarity sorting of driven actin filaments on lipid membranes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mechanical Principles Governing the Shapes of Dendritic Spines.Frontiers in physiology · 2021Article
- Mechanisms of Motor-Independent Membrane Remodeling Driven by Dynamic Microtubules.Current biology : CB · 2020Article
- Induction of membrane curvature by proteins involved in Golgi trafficking.Advances in biological regulation · 2020Review
35 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The elucidation of physical and molecular mechanisms by which a membrane tube is generated from a membrane reservoir is central to the understanding of the structure and dynamics of intracellular organelles and of transport intermediates in eukaryotic cells. Compelling evidence exists that molecular motors of the dynein and kinesin families are involved in the tubulation of organelles. Here, we show that lipid giant unilamellar vesicles (GUVs), to which kinesin molecules have been attached by means of small polystyrene beads, give rise to membrane tubes and to complex tubular networks when incubated in vitro with microtubules and ATP. Similar tubes and networks are obtained with GUVs made of purified Golgi lipids, as well as with Golgi membranes. No tube formation was observed when kinesins were directly bound to the GUV membrane, suggesting that it is critical to distribute the load on both lipids and motors by means of beads. A kinetic analysis shows that network growth occurs in two phases: a phase in which membrane-bound beads move at the same velocity than free beads, followed by a phase in which the tube growth rate decreases and strongly fluctuates. Our work demonstrates that the action of motors bound to a lipid bilayer is sufficient to generate membrane tubes and opens the way to well controlled experiments aimed at the understanding of basic mechanisms in intracellular transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.