ArticleProceedings of the National Academy of Sciences of the United States of America2002
Human antibodies against spores of the genus Bacillus: a model study for detection of and protection against anthrax and the bioterrorist threat.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 60 citations in OpenAlex.
- Naïve Human Antibody Libraries for Infectious Diseases.Advances in experimental medicine and biology · 2017Review
- Quantitative and sensitive RNA based detection of Bacillus spores.Frontiers in microbiology · 2014Article
- Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites.Antimicrobial agents and chemotherapy · 2012Review
- Article
- Beyond natural antibodies: the power of in vitro display technologies.Nature biotechnology · 2011Article
- Ricin detection using phage displayed single domain antibodies.Sensors (Basel, Switzerland) · 2009Article
- Development and implementation of a single-chain Fv antibody for specific detection of Bacillus anthracis spores.Applied and environmental microbiology · 2008Article
- Analysis of epitope information related to Bacillus anthracis and Clostridium botulinum.Expert review of vaccines · 2008Review
- Phase Transition and Optical Properties of DNA-Gold Nanoparticle Assemblies.Plasmonics (Norwell, Mass.) · 2007Article
- Defects can increase the melting temperature of DNA-nanoparticle assemblies.The journal of physical chemistry. B · 2006Article
- Current and developing technologies for monitoring agents of bioterrorism and biowarfare.Clinical microbiology reviews · 2005Review
- An anthrax lethal factor-neutralizing monoclonal antibody protects rats before and after challenge with anthrax toxin.Infection and immunity · 2005Article
- Disorder in DNA-linked gold nanoparticle assemblies.Physical review letters · 2005Article
- A method for the generation of combinatorial antibody libraries using pIX phage display.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A naive, human single-chain Fv (scFv) phage-display library was used in bio-panning against live, native spores of Bacillus subtilis IFO 3336 suspended in solution. A direct in vitro panning and enzyme-linked immunosorbent assay-based selection afforded a panel of nine scFv-phage clones of which two, 5B and 7E, were chosen for further study. These two clones differed in their relative specificity and affinity for spores of B. subtilis IFO 3336 vs. a panel of spores from 11 other Bacillus species/strains. A variety of enzyme-linked immunosorbent assay protocols indicated these scFv-phage clones recognized different spore epitopes. Notably, some spore epitopes markedly changed between the free and microtiter-plate immobilized state as revealed by antibody-phage binding. An additional library selection procedure also was examined by constructing a Fab chain-shuffled sublibrary from the nine positive clones and by using a subtractive panning strategy to remove crossreactivity with B. licheniformis 5A24. The Fab-phage clone 52 was improved compared with 5B and was comparable to 7E in binding B. subtilis IFO 3336 vs. B. licheniformis 5A24, yet showed a distinctive crossreactivity pattern with other spores. We also developed a method to directly detect individual spores by using fluorescently labeled antibody-phage. Finally, a variety of "powders" that might be used in deploying spores of B. anthracis were examined for antibody-phage binding. The strategies described provide a foundation to discover human antibodies specific for native spores of B. anthracis that can be developed as diagnostic and therapeutic reagents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.