ArticleProceedings of the National Academy of Sciences of the United States of America2002
Sustained stimulation shifts the mechanism of endocytosis from dynamin-1-dependent rapid endocytosis to clathrin- and dynamin-2-mediated slow endocytosis in chromaffin cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 123 citations in OpenAlex.
- Persistence of quantal synaptic vesicle recycling in virtual absence of dynamins.The Journal of physiology · 2025Article
- CaEMBO reports · 2022Article
- Concentration of stimulant regulates initial exocytotic molecular plasticity at single cells.Chemical science · 2022Article
- Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Visualization of expanding fusion pores in secretory cells.The Journal of general physiology · 2018Review
- Exocytosis, Endocytosis, and Their Coupling in Excitable Cells.Frontiers in molecular neuroscience · 2017Review
- The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System.Frontiers in molecular neuroscience · 2017Article
- Striatal dopamine neurotransmission: regulation of release and uptake.Basal ganglia · 2016Article
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- Sustained Exocytosis after Action Potential-Like Stimulation at Low Frequencies in Mouse Chromaffin Cells Depends on a Dynamin-Dependent Fast Endocytotic Process.Frontiers in cellular neuroscience · 2016Article
- Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis.The Journal of clinical investigation · 2015Article
- Vesicle Motion during Sustained Exocytosis in Chromaffin Cells: Numerical Model Based on Amperometric Measurements.PloS one · 2015Article
- Recycling endosomes undergo rapid closure of a fusion pore on exocytosis in neuronal dendrites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- A new role for myosin II in vesicle fission.PloS one · 2014Article
- Dynamin-2 function and dysfunction along the secretory pathway.Frontiers in endocrinology · 2013Review
- A nibbling mechanism for clathrin-mediated retrieval of secretory granule membrane after exocytosis.The Journal of biological chemistry · 2013Article
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- Botulinum neurotoxins A and E undergo retrograde axonal transport in primary motor neurons.PLoS pathogens · 2012Article
- MicroRNAs in addiction: adaptation's middlemen?Molecular psychiatry · 2011Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Transient stimulation of secretion in calf chromaffin cells is invariably followed by rapid endocytosis (RE), a clathrin- and K(+)-independent process with a half time of several seconds. Here we show that when exocytosis is triggered in a more sustained manner, a much slower form of endocytosis (SE) replaces RE. SE is complete within 10 min and is abolished when anticlathrin antibodies are introduced into the cell or when intracellular K(+) is removed. RE, but not SE, is blocked by intracellular administration of antidynamin-1 antibodies; the inverse specificity was found for antidynamin-2 antibodies. Replacement of extracellular Ca(2+) by Ba(2+) or Sr(2+) completely blocked RE but had little effect on SE. Thus chromaffin cells exhibit two kinetically and mechanistically distinct forms of endocytosis that are coupled to different extents of exocytosis and are mediated by different isoforms of dynamin. We surmise that RE is associated with the transient fusion ("kiss-and-run") mechanism of transmitter release and is the prevalent means of vesicle recapture and recycling under normal physiological conditions, whereas the clathrin-based SE mechanism comes into play only at higher levels of stimulation and may be associated with complete fusion of vesicles with the plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.