Evidence map›Paper›PMID 11959368›Full record

ArticleRegulatory peptides2002

Expression of pituitary adenylate cyclase-activating polypeptide and PACAP type 1 receptor in the rat gastric and colonic myenteric neurons.

Marcel Miampamba, Patrizia M Germano, Selin Arli, Helen H Wong, David Scott, Yvette Taché, Joseph R Pisegna

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regulatory peptides, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
1.2field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 52 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Review
  7. The Role of Neuropeptides in Mouse Models of Colitis.Journal of molecular neuroscience : MN · 2016
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Pituitary adenylate cyclase-activating polypeptide: a novel peptide with protean implications.Current opinion in endocrinology, diabetes, and obesity · 2007
    Review
  18. Neuroendocrine tumors express PAC1 receptors.Annals of the New York Academy of Sciences · 2006
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Marcel MiampambaCURE: Digestive Diseases Research Center, VA Greater Los Angeles Healthcare System and Department of Medicine, University of California, Los Angeles, 90073, USA.
Patrizia M Germano
Selin Arli
Helen H Wong
David Scott
Yvette Taché
Joseph R Pisegna
University of California, Los Angeles · USVA Greater Los Angeles Healthcare System · US

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
BLRD VA I01 BX004560NIDDK NIH HHS P30 DK041301RRD VA I01 RX000194
6 · The paper itself

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is known to regulate gastric acid secretion and intestinal motility. In the present study, the pattern of distribution of PACAP and PACAP type 1 receptor (PAC1) immunoreactivities were examined in the rat stomach and distal colon using a specific polyclonal antibody raised against rat/human PAC1. Western blot of the membrane preparations of NIH/3T3 cells transfected with the human PAC1 obtained by using rabbit polyclonal anti-PAC1 antibody showed a protein band with a molecular mass of approximately 50 kDa. NIH/3T3 cells transfected with the human PAC1 and incubated with the anti-PAC1 antibody displayed surface cell-type immunoreactivity, which was internalized following ligand exposure. In gastric or colonic longitudinal muscle/myenteric plexus (LMMP) whole mount preparations as well as cryostat sections, PACAP immunoreactivity was observed in cell bodies within the myenteric ganglia and nerve fibers in the muscle layers and mucosa. PAC1 immunoreactivity was confined mainly on the surface of the nerve cells. PACAP and PAC1 immunoreactivities showed a similar pattern of distribution in gastric and colonic tissues. Adjacent sections or LMMP whole mount preparations labeled with protein gene product 9.5 (PGP 9.5) revealed the neuronal identity of myenteric cells bearing PAC1. The neuronal localization of PACAP and PAC1 receptors supports their role in the neural regulation of gastric acid secretion and gastrointestinal motor function.

Indexed as

3T3 CellsAnimalsBlotting, WesternColonEnteric Nervous SystemGastric MucosaGene ExpressionImmunohistochemistryMaleMiceMyenteric PlexusNeuronsNeuropeptidesPituitary Adenylate Cyclase-Activating PolypeptideRatsRats, Sprague-DawleyAdcyap1 protein, mouseAdcyap1 protein, ratAdcyap1r1 protein, mouseAdcyap1r1 protein, ratNeuropeptidesPituitary Adenylate Cyclase-Activating PolypeptideReceptors, Pituitary Adenylate Cyclase-Activating PolypeptideReceptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type IReceptors, Pituitary HormoneThiolester HydrolasesUbiquitin Thiolesterase

Identifiers

PMID11959368
PMCPMC6736535
OpenAlexW1986384712

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.