ArticleThe Journal of physiology2002
Compatible osmolytes modulate the response of porcine endothelial cells to hypertonicity and protect them from apoptosis.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 92 citations in OpenAlex.
- Effects of orange juice and proline betaine on glycine betaine and homocysteine in healthy male subjects.European journal of nutrition · 2007Trial
- Betaine Dietary Supplementation: Healthy Aspects in Human and Animal Nutrition.Antioxidants (Basel, Switzerland) · 2025Review
- Hyperosmotic Stress Induces the Expression of Organic Osmolyte Transporters in Porcine Intestinal Cells and Betaine Exerts a Protective Effect on the Barrier Function.Biomedicines · 2024Article
- Neutral amino acid transporter SLC38A2 protects renal medulla from hyperosmolarity-induced ferroptosis.eLife · 2023Article
- Betaine: A Potential Nutritional Metabolite in the Poultry Industry.Animals : an open access journal from MDPI · 2022Review
- The Role of Betaine in Patients With Chronic Kidney Disease: a Narrative Review.Current nutrition reports · 2022Review
- Effects of Two-Week Betaine Supplementation on Apoptosis, Oxidative Stress, and Aerobic Capacity after Exhaustive Endurance Exercise.Antioxidants (Basel, Switzerland) · 2020Article
- Review
- Review
- Influence of fibre and betaine on development of the gastrointestinal tract of broilers between hatch and 14 d of age.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2019Article
- NFAT5 up-regulates expression of the kidney-specific ubiquitin ligase geneThe Journal of biological chemistry · 2019Article
- The effect of intravitreal injections on dry eye, and proposed management strategies.Clinical ophthalmology (Auckland, N.Z.) · 2017Review
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Hyperosmotic stress induces aquaporin-dependent cell shrinkage, polyphosphate synthesis, amino acid accumulation, and global gene expression changes in Trypanosoma cruzi.The Journal of biological chemistry · 2011Article
- Role of taurine in the vasculature: an overview of experimental and human studies.American journal of cardiovascular disease · 2011Article
- Central pontine myelinolysis: historical and mechanistic considerations.Metabolic brain disease · 2010Article
- Protective effects of taurine on endothelial cells impaired by high glucose and oxidized low density lipoproteins.European journal of nutrition · 2007Article
- Pegylated interferon-alpha plus taurine in treatment of rat liver fibrosis.World journal of gastroenterology · 2007Article
- Hyperosmotic stress response: comparison with other cellular stresses.Pflugers Archiv : European journal of physiology · 2007Review
- Creatine as a compatible osmolyte in muscle cells exposed to hypertonic stress.The Journal of physiology · 2006Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Porcine pulmonary arterial endothelial cells accumulated myo-inositol and taurine, as well as betaine, during adaptation to hypertonic stress. The cells grew and maintained their normal morphology during culture in hypertonic (0.5 osmol (kg H(2)O)(-1)) medium that contained osmolytes such as betaine, myo-inositol or taurine at concentrations close to reported physiological values. The cells did not grow well in hypertonic medium depleted of potential compatible osmolytes. After a few days, cell density decreased by about 50 % and many cells rounded up and detached from the plates, their nuclei showing clear apoptotic morphology. The caspase-3 activity of the cells also increased dramatically under these conditions, but remained negligibly low when betaine and myo-inositol were added to the medium. Addition of betaine and myo-inositol to hypertonic medium depleted of compatible osmolytes increased the number of colonies remaining after 12 days of culture; with each solute at 30-100 micromol l(-1) the number increased about sixfold. In the absence of compatible osmolytes, increased mRNA levels and corresponding activities of betaine/gamma-aminobutyric acid transporter (BGT1) and sodium/myo-inositol transporter (SMIT) induced by hypertonicity remained high after 72 h incubation, whereas they were down regulated in the presence of betaine and myo-inositol. Similarly, the down regulation of the amino acid System A transporter (ATA2) was markedly slowed in the absence of compatible osmolytes. We conclude that these compatible osmolytes at concentrations close to physiological values enable the endothelial cells to adapt to hypertonic stress, protecting them from apoptosis, and also modulate the adaptation process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.