ArticleThe Biochemical journal2002
Aerobic sn-glycerol-3-phosphate dehydrogenase from Escherichia coli binds to the cytoplasmic membrane through an amphipathic alpha-helix.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Article
- Riboflavin salvage by Borrelia burgdorferi supports carbon metabolism and is essential for survival in the tick vector.Molecular microbiology · 2022Article
- Reverse Electron Transfer by Respiratory Complex I Catalyzed in a Modular Proteoliposome System.Journal of the American Chemical Society · 2022Article
- Dual-function AzuCR RNA modulates carbon metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The FAD-dependent glycerol-3-phosphate dehydrogenase of Giardia duodenalis: an unconventional enzyme that interacts with the g14-3-3 and it is a target of the antitumoral compound NBDHEX.Frontiers in microbiology · 2015Article
- Proteome-wide subcellular topologies of E. coli polypeptides database (STEPdb).Molecular & cellular proteomics : MCP · 2014Article
- The Escherichia coli peripheral inner membrane proteome.Molecular & cellular proteomics : MCP · 2013Article
- Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli.Microbial cell factories · 2013Article
- Application of universal stress proteins in probing the dynamics of potent degraders in complex terephthalate metagenome.BioMed research international · 2013Article
- Escherichia coli strains engineered for homofermentative production of D-lactic acid from glycerol.Applied and environmental microbiology · 2010Article
- Anaerobic fermentation of glycerol in Paenibacillus macerans: metabolic pathways and environmental determinants.Applied and environmental microbiology · 2009Article
- Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals.Applied and environmental microbiology · 2008Article
- Mutation in glycerol-3-phosphate dehydrogenase 1 like gene (GPD1-L) decreases cardiac Na+ current and causes inherited arrhythmias.Circulation · 2007Article
- In vitro synthesis of minus-strand RNA by an isolated cereal yellow dwarf virus RNA-dependent RNA polymerase requires VPg and a stem-loop structure at the 3' end of the virus RNA.Journal of virology · 2006Article
- Identification of a region of the tobacco mosaic virus 126- and 183-kilodalton replication proteins which binds specifically to the viral 3'-terminal tRNA-like structure.Journal of virology · 2003Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
sn-Glycerol-3-phosphate dehydrogenase (GlpD) from Escherichia coli is a peripheral membrane enzyme involved in respiratory electron transfer. For it to display its enzymic activity, binding to the inner membrane is required. The way the enzyme interacts with the membrane and how this controls activity has not been elucidated. In the present study we provide evidence for direct protein-lipid interaction. Using the monolayer technique, we observed insertion of GlpD into lipid monolayers with a clear preference for anionic phospholipids. GlpD variants with point mutations in their predicted amphipathic helices showed a decreased ability to penetrate anionic phospholipid monolayers. From these data we propose that membrane binding of GlpD occurs by insertion of an amphipathic helix into the acyl-chain region of lipids mediated by negatively charged phospholipids.
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