ArticleInfection and immunity2002
Identification and cloning of a cryptococcal deacetylase that produces protective immune responses.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Acetylation and accessibility ofCell surface (Amsterdam, Netherlands) · 2026Article
- Pathogenicity and virulence of Cryptococcus neoformans from an environmental perspective.Virulence · 2025Review
- Fungal Treatment for the Valorization of Technical Soda Lignin.Journal of fungi (Basel, Switzerland) · 2021Article
- Unique subsite specificity and potential natural function of a chitosan deacetylase from the human pathogenProceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Call to Arms: Quest for a Cryptococcal Vaccine.Trends in microbiology · 2018Review
- Review
- Glucosylceramide Administration as a Vaccination Strategy in Mouse Models of Cryptococcosis.PloS one · 2016Article
- Virulence-Associated Enzymes of Cryptococcus neoformans.Eukaryotic cell · 2015Review
- Article
- Mannoprotein MP84 mediates the adhesion of Cryptococcus neoformans to epithelial lung cells.Frontiers in cellular and infection microbiology · 2014Article
- Modular nanotransporters: a multipurpose in vivo working platform for targeted drug delivery.International journal of nanomedicine · 2012Article
- Article
- A proteomic-based approach for the identification of immunodominant Cryptococcus neoformans proteins.Proteomics · 2009Article
- Loss of allergen 1 confers a hypervirulent phenotype that resembles mucoid switch variants of Cryptococcus neoformans.Infection and immunity · 2009Article
- Chitosan, the deacetylated form of chitin, is necessary for cell wall integrity in Cryptococcus neoformans.Eukaryotic cell · 2007Article
- Protective immunity against cryptococcus neoformans infection.McGill journal of medicine : MJM : an international forum for the advancement of medical sciences by students · 2007Article
- Characterization of two novel cryptococcal mannoproteins recognized by immune sera.Infection and immunity · 2005Article
- Protective efficacy of antigenic fractions in mouse models of cryptococcosis.Infection and immunity · 2004Article
- Induction of T helper type 1 responses by a polysaccharide deacetylase from Cryptococcus neoformans.Infection and immunity · 2003Article
- Purification and characterization of a second immunoreactive mannoprotein from Cryptococcus neoformans that stimulates T-Cell responses.Infection and immunity · 2002Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Cell-mediated immunity plays a crucial role in host defenses against Cryptococcus (Filobasidiella) neoformans. Therefore, the identification of cryptococcal antigens capable of producing T-cell-mediated responses, such as delayed-type hypersensitivity (DTH) reactions, may be useful in the development of immune-based strategies to control cryptococcosis. In order to characterize DTH-producing antigens, culture supernatants from the unencapsulated Cap-67 strain were separated by anion-exchange chromatography. After further fractionation by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis, a purified protein with an apparent molecular mass of 25 kDa was found to produce DTH, as evidenced by increased footpad swelling in mice immunized with culture supernatants, relative to unimmunized mice. The 20-amino-acid N-terminal sequence of the 25-kDa protein was used to search data of the C. neoformans Genome Project. Based on the genomic DNA sequence, a DNA probe was used to screen a lambda cDNA library prepared from strain B3501. Clones were isolated containing the full-length gene (d25), which showed homology with a number of polysaccharide deacetylases from fungi and bacteria. The recombinant d25 protein expressed in Escherichia coli was similar to the natural one in DTH-producing activity. Moreover, immunization with either the natural or the recombinant protein prolonged survival and decreased fungal burden in mice challenged with the highly virulent C. neoformans strain H99. In conclusion, we have described the first cryptococcal gene whose product, a 25-kDa extracellular polysaccharide deacetylase, has been shown to induce protective immunity responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.