ArticleMolecular biology of the cell2002
Kinesin-II is required for flagellar sensory transduction during fertilization in Chlamydomonas.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 75 citations in OpenAlex.
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- A cytoplasmic protein kinase couples engagement of Chlamydomonas ciliary receptors to cAMP-dependent cellular responses.Journal of cell science · 2022Article
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Sexual reproduction and sex determination in green algae.Journal of plant research · 2017Review
- The ciliary inner dynein arm, I1 dynein, is assembled in the cytoplasm and transported by IFT before axonemal docking.Cytoskeleton (Hoboken, N.J.) · 2014Article
- How do cilia organize signalling cascades?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
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- Regulated membrane protein entry into flagella is facilitated by cytoplasmic microtubules and does not require IFT.Current biology : CB · 2013Article
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- The future of ciliary and flagellar membrane research.Molecular biology of the cell · 2012Article
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- Heterotrimeric kinesin-II is necessary and sufficient to promote different stepwise assembly of morphologically distinct bipartite cilia in Drosophila antenna.Molecular biology of the cell · 2011Article
- The ciliary membrane.Current opinion in cell biology · 2010Review
- Hearing in Drosophila requires TilB, a conserved protein associated with ciliary motility.Genetics · 2010Article
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- Genetic and phenotypic analysis of flagellar assembly mutants in Chlamydomonas reinhardtii.Methods in cell biology · 2009Article
- High-resolution crystal structure and in vivo function of a kinesin-2 homologue in Giardia intestinalis.Molecular biology of the cell · 2008Article
- Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella.The Journal of cell biology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The assembly and maintenance of eucaryotic flagella and cilia depend on the microtubule motor, kinesin-II. This plus end-directed motor carries intraflagellar transport particles from the base to the tip of the organelle, where structural components of the axoneme are assembled. Here we test the idea that kinesin-II also is essential for signal transduction. When mating-type plus (mt+) and mating-type minus (mt-) gametes of the unicellular green alga Chlamydomonas are mixed together, binding interactions between mt+ and mt- flagellar adhesion molecules, the agglutinins, initiate a signaling pathway that leads to increases in intracellular cAMP, gamete activation, and zygote formation. A critical question in Chlamydomonas fertilization has been how agglutinin interactions are coupled to increases in intracellular cAMP. Recently, fla10 gametes with a temperature-sensitive defect in FLA10 kinesin-II were found to not form zygotes at the restrictive temperature (32 degrees C). We found that, although the rates and extents of flagellar adhesion in fla10 gametes at 32 degrees C are indistinguishable from wild-type gametes, the cells do not undergo gamete activation. On the other hand, fla10 gametes at 32 degrees C regulated agglutinin location and underwent gamete fusion when the cells were incubated in dibutyryl cAMP, indicating that their capacity to respond to the cAMP signal was intact. We show that the cellular defect in the fla10 gametes at 32 degrees C is a failure to undergo increases in cAMP during flagella adhesion. Thus, in addition to being essential for assembly and maintenance of the structural components of flagella, kinesin-II/intraflagellar transport plays a role in sensory transduction in these organelles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.