ArticleMolecular biology of the cell2002
Morphology and dynamics of the endocytic pathway in Dictyostelium discoideum.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 75 citations in OpenAlex.
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- Starvation Induces Extracellular Accumulation of Polyphosphate inInternational journal of molecular sciences · 2023Article
- IKKγ/NEMO Localization into Multivesicular Bodies.International journal of molecular sciences · 2022Article
- From Pinocytosis to Methuosis-Fluid Consumption as a Risk Factor for Cell Death.Frontiers in cell and developmental biology · 2021Review
- The Polarized Redistribution of the Contractile Vacuole to the Rear of the Cell is Critical for Streaming and is Regulated by PI(4,5)P2-Mediated Exocytosis.Frontiers in cell and developmental biology · 2021Review
- A Two-Armed Probe for In-Cell DEER Measurements on Proteins*.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Ubiquitous macropinocytosis in anthozoans.eLife · 2020Article
- Heterogeneities Shape Passive Intracellular Transport.Biophysical journal · 2019Article
- The origins and evolution of macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Role of inositol polyphosphates in programed cell death in Dictyostelium discoideum and its developmental life cycle.Molecular and cellular biochemistry · 2018Article
- Eat Prey, Live:Frontiers in immunology · 2017Review
- Article
- Nutrient-sensing mechanisms and pathways.Nature · 2015Review
- Binding and Endocytosis of Bovine Hololactoferrin by the Parasite Entamoeba histolytica.BioMed research international · 2015Article
- WASH is required for lysosomal recycling and efficient autophagic and phagocytic digestion.Molecular biology of the cell · 2013Article
- Campylobacter jejuni actively invades the amoeba Acanthamoeba polyphaga and survives within non digestive vacuoles.PloS one · 2013Article
- The balance in the delivery of ER components and the vacuolar proton pump to the phagosome depends on myosin IK in Dictyostelium.Molecular & cellular proteomics : MCP · 2012Article
- Rab8a regulates the exocyst-mediated kiss-and-run discharge of the Dictyostelium contractile vacuole.Molecular biology of the cell · 2012Article
- Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis.The Journal of cell biology · 2011Article
- Endocytosis-like protein uptake in the bacterium Gemmata obscuriglobus.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dictyostelium discoideum is a genetically and biochemically tractable social amoeba belonging to the crown group of eukaryotes. It performs some of the tasks characteristic of a leukocyte such as chemotactic motility, macropinocytosis, and phagocytosis that are not performed by other model organisms or are difficult to study. D. discoideum is becoming a popular system to study molecular mechanisms of endocytosis, but the morphological characterization of the organelles along this pathway and the comparison with equivalent and/or different organelles in animal cells and yeasts were lagging. Herein, we used a combination of evanescent wave microscopy and electron microscopy of rapidly frozen samples to visualize primary endocytic vesicles, vesicular-tubular structures of the early and late endo-lysosomal system, such as multivesicular bodies, and the specialized secretory lysosomes. In addition, we present biochemical and morphological evidence for the existence of a micropinocytic pathway, which contributes to the uptake of membrane along side macropinocytosis, which is the major fluid phase uptake process. This complex endosomal compartment underwent continuous cycles of tubulation/vesiculation as well as homo- and heterotypic fusions, in a way reminiscent of mechanisms and structures documented in leukocytes. Finally, egestion of fluid phase from the secretory lysosomes was directly observed.
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