ArticleMolecular biology of the cell2002
Dopamine-induced exocytosis of Na,K-ATPase is dependent on activation of protein kinase C-epsilon and -delta.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 95 citations in OpenAlex.
- The Effects of the Natriuretic Peptide System on Alveolar Epithelium in Heart Failure.International journal of molecular sciences · 2025Article
- Identified and potential internalization signals involved in trafficking and regulation of NaMolecular and cellular biochemistry · 2024Review
- Ecstasy metabolites and monoamine neurotransmitters upshift the NaArchives of toxicology · 2022Article
- Differential expression and characterization of ATP1A1 exon17 gene by high resolution melting analysis and RT-PCR in Indian goats.Molecular biology reports · 2019Article
- DR region of NaScientific reports · 2018Article
- Lung Edema Clearance: Relevance to Patients with Lung Injury.Rambam Maimonides medical journal · 2015Article
- Renal dopaminergic system: Pathophysiological implications and clinical perspectives.World journal of nephrology · 2015Review
- Article
- Developmental differences in hyperoxia-induced oxidative stress and cellular responses in the murine lung.Free radical biology & medicine · 2013Article
- Protein Kinase C Inhibitors as Modulators of Vascular Function and their Application in Vascular Disease.Pharmaceuticals (Basel, Switzerland) · 2013Article
- NADPH oxidase and PKC contribute to increased Na transport by the thick ascending limb during type 1 diabetes.Hypertension (Dallas, Tex. : 1979) · 2012Article
- Cholinergic signaling inhibits oxalate transport by human intestinal T84 cells.American journal of physiology. Cell physiology · 2012Article
- Elevated CO(2) levels cause mitochondrial dysfunction and impair cell proliferation.The Journal of biological chemistry · 2011Article
- Targeted mutations in the Na,K-ATPase α 2 isoform confer ouabain resistance and result in abnormal behavior in mice.Synapse (New York, N.Y.) · 2011Article
- Hypoxia. 2. Hypoxia regulates cellular metabolism.American journal of physiology. Cell physiology · 2011Review
- AS160 associates with the Na+,K+-ATPase and mediates the adenosine monophosphate-stimulated protein kinase-dependent regulation of sodium pump surface expression.Molecular biology of the cell · 2010Article
- Extracellular signal-regulated kinase (ERK) participates in the hypercapnia-induced Na,K-ATPase downregulation.FEBS letters · 2010Article
- Insulin regulates alveolar epithelial function by inducing Na+/K+-ATPase translocation to the plasma membrane in a process mediated by the action of Akt.Journal of cell science · 2010Article
- Ubiquitination participates in the lysosomal degradation of Na,K-ATPase in steady-state conditions.American journal of respiratory cell and molecular biology · 2009Article
- Myosin-Va restrains the trafficking of Na+/K+-ATPase-containing vesicles in alveolar epithelial cells.Journal of cell science · 2009Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
The purpose of this study was to define mechanisms by which dopamine (DA) regulates the Na,K-ATPase in alveolar epithelial type 2 (AT2) cells. The Na,K-ATPase activity increased by twofold in cells incubated with either 1 microM DA or a dopaminergic D(1) agonist, fenoldopam, but not with the dopaminergic D(2) agonist quinpirole. The increase in activity paralleled an increase in Na,K-ATPase alpha1 and beta1 protein abundance in the basolateral membrane (BLM) of AT2 cells. This increase in protein abundance was mediated by the exocytosis of Na,K-pumps from late endosomal compartments into the BLM. Down-regulation of diacylglycerol-sensitive types of protein kinase C (PKC) by pretreatment with phorbol 12-myristate 13-acetate or inhibition with bisindolylmaleimide prevented the DA-mediated increase in Na,K-ATPase activity and exocytosis of Na,K-pumps to the BLM. Preincubation of AT2 cells with either 2-[1-(3-dimethylaminopropyl)-5-methoxyindol-3-yl]-3-(1H-indol-3-yl)maleimide (Gö6983), a selective inhibitor of PKC-delta, or isozyme-specific inhibitor peptides for PKC-delta or PKC-epsilon inhibited the DA-mediated increase in Na,K-ATPase. PKC-delta and PKC-epsilon, but not PKC-alpha or -beta, translocated from the cytosol to the membrane fraction after exposure to DA. PKC-delta- and PKC-epsilon-specific peptide agonists increased Na,K-ATPase protein abundance in the BLM. Accordingly, dopamine increased Na,K-ATPase activity in alveolar epithelial cells through the exocytosis of Na,K-pumps from late endosomes into the basolateral membrane in a mechanism-dependent activation of the novel protein kinase C isozymes PKC-delta and PKC-epsilon.
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