ArticleMolecular biology of the cell2002
Phosphoinositides regulate membrane-dependent actin assembly by latex bead phagosomes.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 70 citations in OpenAlex.
- Physiological roles of phosphoinositides and inositol phosphates: Implications for metabolic dysfunction-associated steatotic liver disease.Clinical science (London, England : 1979) · 2025Review
- An In Vitro System to Analyze Generation and Degradation of Phagosomal Phosphatidylinositol Phosphates.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Better Together: Current Insights Into Phagosome-Lysosome Fusion.Frontiers in immunology · 2021Review
- Expression and Functional Role of TRPV4 in Bone Marrow-Derived CD11cInternational journal of molecular sciences · 2019Article
- Osmotic activation of a CaPhysiological reports · 2017Article
- Macrophage-Bacteria Interactions-A Lipid-Centric Relationship.Frontiers in immunology · 2017Review
- U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C.SpringerPlus · 2016Article
- Phosphatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate regulate phagolysosome biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Ezrin-related Phosphoinositide pathway modifies RhoA and Rac1 in human osteosarcoma cell lines.Journal of cell communication and signaling · 2015Article
- Ezrin silencing remodulates the expression of Phosphoinositide-specific Phospholipase C enzymes in human osteosarcoma cell lines.Journal of cell communication and signaling · 2014Article
- Piperine inhibits type II phosphatidylinositol 4-kinases: a key component in phosphoinositides turnover.Molecular and cellular biochemistry · 2014Article
- Gelsolin and non-muscle myosin IIA interact to mediate calcium-regulated collagen phagocytosis.The Journal of biological chemistry · 2011Article
- The platelet actin cytoskeleton associates with SNAREs and participates in alpha-granule secretion.Biochemistry · 2010Article
- TRPV2 has a pivotal role in macrophage particle binding and phagocytosis.Nature immunology · 2010Article
- Actin dynamics and Rho GTPases regulate the size and formation of parasitophorous vacuoles containing Coxiella burnetii.Infection and immunity · 2009Article
- The protein kinase A-anchoring protein moesin is bound to pigment granules in melanophores.Traffic (Copenhagen, Denmark) · 2009Article
- Actin and phosphoinositide recruitment to fully formed Candida albicans phagosomes in mouse macrophages.Journal of innate immunity · 2009Article
- Modelling phagosomal lipid networks that regulate actin assembly.BMC systems biology · 2008Article
- Mammalian septins are required for phagosome formation.Molecular biology of the cell · 2008Article
- Four distinct pathways of hemoglobin uptake in the malaria parasite Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin assembly on membrane surfaces is an elusive process in which several phosphoinositides (PIPs) have been implicated. We have reconstituted actin assembly using a defined membrane surface, the latex bead phagosome (LBP), and shown that the PI(4,5)P(2)-binding proteins ezrin and/or moesin were essential for this process (). Here, we provide several lines of evidence that both preexisting and newly synthesized PI(4,5)P(2), and probably PI(4)P, are essential for phagosomal actin assembly; only these PIPs were routinely synthesized from ATP during in vitro actin assembly. Treatment of LBP with phospholipase C or with adenosine, an inhibitor of type II PI 4-kinase, as well as preincubation with anti-PI(4)P or anti-PI(4,5)P(2) antibodies all inhibited this process. Incorporation of extra PI(4)P or PI(4,5)P(2) into the LBP membrane led to a fivefold increase in the number of phagosomes that assemble actin. An ezrin mutant mutated in the PI(4,5)P(2)-binding sites was less efficient in binding to LBPs and in reconstituting actin assembly than wild-type ezrin. Our data show that PI 4- and PI 5-kinase, and under some conditions also PI 3-kinase, activities are present on LBPs and can be activated by ATP, even in the absence of GTP or cytosolic components. However, PI 3-kinase activity is not required for actin assembly, because the process was not affected by PI 3-kinase inhibitors. We suggest that the ezrin-dependent actin assembly on the LBP membrane may require active turnover of D4 and D5 PIPs on the organelle membrane.
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