ArticleGene therapy2002
Enhanced transgene expression in primitive hematopoietic progenitor cells and embryonic stem cells efficiently transduced by optimized retroviral hybrid vectors.
Article in Gene therapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 96 citations in OpenAlex.
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- The collectrin-like part of the SARS-CoV-1 and -2 receptor ACE2 is shed by the metalloproteinases ADAM10 and ADAM17.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
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- The iRhom homology domain is indispensable for ADAM17-mediated TNFα and EGF receptor ligand release.Cellular and molecular life sciences : CMLS · 2021Article
- ADAM17-triggered TNF signalling protects the ageing Drosophila retina from lipid droplet-mediated degeneration.The EMBO journal · 2020Article
- Deciphering site 3 interactions of interleukin 12 and interleukin 23 with their cognate murine and human receptors.The Journal of biological chemistry · 2020Article
- Synthetic cytokine receptors transmit biological signals using artificial ligands.Nature communications · 2018Article
- The length of the interleukin-11 receptor stalk determines its capacity for classic signaling.The Journal of biological chemistry · 2018Article
- Identification of Kinases and Phosphatases That Regulate ATG4B Activity by siRNA and Small Molecule Screening in Cells.Frontiers in cell and developmental biology · 2018Article
- Cancer cell specific inhibition of Wnt/β-catenin signaling by forced intracellular acidification.Cell discovery · 2018Article
- IL-6/IL-12 Cytokine Receptor Shuffling of Extra- and Intracellular Domains Reveals Canonical STAT Activation via Synthetic IL-35 and IL-39 Signaling.Scientific reports · 2017Article
- GFP-Grb2 Translocation Assay Using High-content Imaging to Screen for Modulators of EGFR-signaling.Bio-protocol · 2017Article
- Synthetic Deletion of the Interleukin 23 Receptor (IL-23R) Stalk Region Led to Autonomous IL-23R Homodimerization and Activation.Molecular and cellular biology · 2017Article
- Protein abundance of AKT and ERK pathway components governs cell type-specific regulation of proliferation.Molecular systems biology · 2017Article
- Proteolytic Origin of the Soluble Human IL-6R In Vivo and a Decisive Role of N-Glycosylation.PLoS biology · 2017Article
- Ribose 5-phosphate isomerase inhibits LC3 processing and basal autophagy.Cellular signalling · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oncoretroviral vectors have been successfully used in gene therapy trials, yet low transduction rates and loss of transgene expression are still major obstacles for their application. To overcome these problems we modified the widely used Moloney murine leukemia virus-derived retroviral vector pMX by replacing the 3'LTR with the spleen focus-forming virus LTR and inserting the woodchuck hepatitis B virus post-translational regulatory element. To compare requirements crucial for efficient transgene expression, we generated the hybrid retroviral vectors pMOWS and pOWS that harbor the complete murine embryonic stem cell virus (MESV)-leader sequence or a shortened MESV-leader not comprising primer binding site (PBS) and splice donor (SD). Applying these retroviral vectors significantly augmented transgene expression in hematopoietic cell lines and progenitor cells. For transduction of murine embryonic stem (ES) cells the retroviral vector pMOWS that harbors the MESV-PBS and -SD was superior resulting in 65% green fluorescent protein (GFP) expressing ES cells. Surprisingly, in murine and human primitive hematopoietic progenitor cells (HPC), the highest efficiency of up to 66% GFP expressing cells was achieved with pOWS, a retroviral vector that retains the negative regulatory element coinciding with the MoMuLV-PBS. In summary our hybrid retroviral vectors facilitate significantly improved transgene expression in multipotent cells and thus possess great potential for reconstituting genes in primary cells of disease models, as well as for gene therapy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.