ArticleMolecular and cellular biology2002
The MyoD-inducible p204 protein overcomes the inhibition of myoblast differentiation by Id proteins.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 84 citations in OpenAlex.
- EZH2 modulates retinoic acid signaling to ensure myotube formation during development.FEBS letters · 2022Article
- Unresolved intramuscular inflammation, not diminished skeletal muscle regenerative capacity, is at the root of rheumatoid cachexia: insights from a rat CIA model.Physiological reports · 2021Article
- New Insights into the Development and Morphogenesis of the Cardiac Purkinje Fiber Network: Linking Architecture and Function.Journal of cardiovascular development and disease · 2021Review
- The interferon-inducible protein p202 promotes osteogenesis in mouse bone marrow stromal cells.Bioscience reports · 2018Article
- RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity.Scientific reports · 2018Article
- p204 Is Required for Canonical Lipopolysaccharide-induced TLR4 Signaling in Mice.EBioMedicine · 2018Article
- The Id-protein family in developmental and cancer-associated pathways.Cell communication and signaling : CCS · 2017Review
- Regulatory roles of interferon-inducible protein 204 on differentiation and vasculogenic activity of endothelial progenitor cells.Stem cell research & therapy · 2016Article
- Comparative transcriptome analysis of muscular dystrophy models Large(myd), Dmd(mdx)/Large(myd) and Dmd(mdx): what makes them different?European journal of human genetics : EJHG · 2016Article
- The roles of interferon-inducible p200 family members IFI16 and p204 in innate immune responses, cell differentiation and proliferation.Genes & diseases · 2015Article
- Wanderings in biochemistry.The Journal of biological chemistry · 2014Article
- GEP constitutes a negative feedback loop with MyoD and acts as a novel mediator in controlling skeletal muscle differentiation.Cellular and molecular life sciences : CMLS · 2012Article
- Suppression of BMP-Smad signaling axis-induced osteoblastic differentiation by small C-terminal domain phosphatase 1, a Smad phosphatase.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Inhibitor of DNA binding 4 (ID4) regulation of adipocyte differentiation and adipose tissue formation in mice.The Journal of biological chemistry · 2010Article
- Dual roles of smad proteins in the conversion from myoblasts to osteoblastic cells by bone morphogenetic proteins.The Journal of biological chemistry · 2010Article
- Interaction between cartilage oligomeric matrix protein and extracellular matrix protein 1 mediates endochondral bone growth.Matrix biology : journal of the International Society for Matrix Biology · 2010Article
- The p200 family protein p204 as a modulator of cell proliferation and differentiation: a brief survey.Cellular and molecular life sciences : CMLS · 2010Review
- Sequestration of E12/E47 and suppression of p27KIP1 play a role in Id2-induced proliferation and tumorigenesis.Carcinogenesis · 2009Article
- FHL2 interacts with and acts as a functional repressor of Id2 in human neuroblastoma cells.Nucleic acids research · 2009Article
- The intracellular localization of ID2 expression has a predictive value in non small cell lung cancer.PloS one · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The murine p204 protein level is highest in heart and skeletal muscle. During the fusion of cultured myoblasts to myotubes, the p204 level increases due to transcription dependent on the muscle-specific MyoD protein, and p204 is phosphorylated and translocated from the nucleus to the cytoplasm. p204 overexpression accelerates myoblast fusion in differentiation medium and triggers this process even in growth medium. Here we report that p204 is required for the differentiation of C2C12 myoblasts. We propose that it enables the differentiation, at least in part, by overcoming the inhibition of the activities of the MyoD and E47 proteins by the Id proteins: Id1, Id2, and Id3. These are known to inhibit skeletal muscle differentiation by binding and blocking the activity of MyoD, E12/E47, and other myogenic basic helix-loop-helix (bHLH) proteins. Our hypothesis is based on the following findings. (i) A decrease in the p204 level in C2C12 myoblasts by antisense RNA (a) increased the level of the Id2; (b) inhibited the MyoD-, E12/E47-, and other bHLH protein-dependent accumulation of the muscle-specific myosin heavy-chain protein; and (c) inhibited the fusion of myoblasts to myotubes in differentiation medium. (ii) p204 bound to the Id proteins in vitro and in vivo. (iii) In the binding of p204 to Id2, the b segment of p204 and the HLH segment of Id2 were involved. (iv) Addition of p204 overcame the inhibition by the Id proteins of the binding of MyoD and E47 to DNA in vitro. (v) Overexpression of p204 in myoblasts (a) decreased the level of the Id proteins, even in a culture in growth medium, and (b) overcame the inhibition by the Id proteins of MyoD- and E47 dependent transcription and also overcame the inhibition by Id2 of the fusion of myoblasts to myotubes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.